Suprex Corp.

Vascor, Inc.

Scientific Industries, Inc.

Other

Fisher Scientific Company

Automated Cell, Inc.

Kairos Instruments, LLC

Douglas J Koebler

Raymond K Houck

Glen P Williams

Marlin S Heilman

David M Reilly

Joseph G Cremonese

Jon David Wagner

Joseph M Levy

Michael Tolosa

Alfred Blalock Bahnson

Charles Robert Kohler

Victor G Danielson

Brookman P March

William Chandler

Robert Dale Parks

Edward Karl Prem

David J Brigati

Daniel Richard Moore

Carlo Edward Cuomo

Kenneth J Kato

Paul A Bauer, Jr

Lei Qian

Charalambos N Arthanassiou

Kris Sachsenmeier

Embodiments of a cell culture monitoring system are disclosed that may include a device having collimating and filtering components to elicit and detect fluorescence from a substrate located within a reaction vessel. The device may be used to detect changes in pH and dissolved oxygen levels in a liquid contained in the reaction vessel due to growth of living cells. Excitation light beams can be generated and collimated by a beam combiner and directed into the reaction vessel so as to be incident upon the substrate to cause the substrate to fluoresce. Some embodiments include use of expected wavelength offsets and shifted filters/mirrors to improve functionality and reduce space of device components.

Apparatus for detecting pH and dissolved oxygen

A system for assisting blood flow in a patient includes an implantable pump system for assisting in blood flow from the heart and an implantable control system for communicative connection with the implantable pump system. The control system includes a pacemaker system that is operative to control heart rate and the pump system interdependently to achieve a determined level of blood flow assist. The control system is adapted to increase the heart rate of the patient to above a normal heart rate for the patient under the present conditions of the patient via the pacemaker while controlling the pump system to achieve the determined level of blood flow assist based upon the increased heart rate and a level of assist provided by the pump system.

Control of blood flow assist systems

An optical microscope is provided with an adjustable optical phase ring. The adjustable ring provides a way to compensate for distortion in the visible phase ring before the light reaches the sample. In an inverted microscope, when observing transparent cells under a liquid, the visible light phase ring is distorted. By the use of a Liquid Crystal Display (LCD) in place of a fixed ring, the projected ring is adjusted to realign the light and produce phase. In a typical micro plate, the meniscus formed produces a lens effect that is realigned by providing changes in the position and pattern, to allow phase imaging over a wider portion of the well. The realignment of the ring can be manual or automated and can be dynamically adjusted based upon an observed image of the sample.

Adaptive phase contrast microscope

In one aspect, an implantable pump system for assisting blood flow in a patient includes a blood flow path comprising a flexible conduit, at least one valve in fluid connection with the flexible conduit, a drive system in operative connection with the valve to move the valve in a reciprocating manner, an implantable control system and at least one sensor in communicative connection with the control system. The sensor is adapted to measure at least one property of blood. The control system is adapted to control movement of the valve on the basis of data regarding position of the valve with respect to time or a derivative thereof (that is, position, velocity, acceleration or a derivative thereof) in combination with data of the measured at least one property of blood from the sensor.

A pump includes a flexible conduit, at least one valve attached to the flexible conduit about the perimeter of the valve; and a drive mechanism to move the valve to pump blood within the conduit. The drive mechanism can, for example, be adapted to complete a single stroke during each heart ventricle contraction and/or to complete multiple strokes (that is, oscillate) during a single contraction. The moveable valve includes a plurality of openings. Each of the plurality of openings has a closure mechanism in operative connection therewith which is operable to at least partially close the opening to which it is operatively connected when the moveable valve is moved forward and to open the opening to which it is operatively connected when the valve is moved rearward. In one embodiment, each closure mechanism includes a flap of resilient material.

Devices, systems and methods for assisting blood flow

An apparatus for monitoring proteins and cells. The apparatus includes a plate having wells in which cells are disposed. The apparatus includes means for analyzing the effect or proteins and other biological and chemical moieties on the cells. A method for monitoring of proteins and cells. A method for analyzing a cells. An apparatus for aligning light in a well of a plate for holding cells. A method for lighting a well. A method for determining a condition of a cell. An apparatus for indicating a condition of a cell. A method for establishing a focus profile of a plate having wells for holding cells. A method for manipulating cells. An apparatus for manipulating cells.

Method and apparatus for monitoring of proteins and cells

The present invention pertains to an apparatus for gathering analyte. The apparatus comprises a pumping mechanism for pumping solvent at supercritical pressure. The apparatus also comprises an extraction mechanism which receives a first sample and at least a second sample separate and distinct from the first sample and extract analyte from them essentially simultaneously. The extraction mechanism is connected to the pump mechanism to receive solvent at supercritical pressure for extracting the analyte from the samples. The apparatus additionally comprises a collection mechanism for collecting analyte from the solvent after the analyte has been extracted from the samples. The collection mechanism receives the supercritical solvent having the analyte extracted from the samples from the extraction mechanism. The present invention pertains to a method for extracting analyte. The method comprises the steps of pumping solvent at a supercritical pressure. Then there is the step of extracting analyte from N samples essentially simultaneously with the solvent at a supercritical pressure, where N is greater than or equal to two and is an integer. The N samples are separate and distinct from each other. Then there is the step of collecting the analyte of the N samples from the solvent.

Method and apparatus for determination of analyte concentration

A supercritical fluid extraction (SFE) system. The SFE system includes a mechanism for providing supercritical fluid for extracting analyte from a sample. The SFE system also includes an extraction vessel and a mechanism for moving the extraction vessel into fluidic communication with the SFE system so that supercritical fluid can flow through the extraction vessel and extract analyte from the sample. Preferably, the SFE system is additionally comprised of a mechanism for holding a plurality of extraction vessels and an extraction chamber assembly. In this design, the moving mechanism selectively moves an extraction vessel from the holding mechanism into the extraction chamber in fluidic communication with the SFE system. Preferably, the SFE system further includes a computer for controlling the functions of the SFE system. The computer, for instance, could automatically and sequentially control the supercritical fluid extractions of the samples within the holding mechanism. The SFE system preferably includes a mechanism for collecting analyte from the fluid with analyte. The collecting mechanism is in fluid communication with extraction chamber assembly and preferably includes a restrictor for controlling the flow of fluid with analyte to the collecting mechanism. The restrictor converts the fluid with analyte from a first supercritical pressure to a second pressure which is less than the first supercritical pressure.

Automated supercritical fluid extraction method and apparatus

The present invention is an apparatus for restricting the flow of fluid. The apparatus defines a tubular member having a channel. The tubular member has a first portion in which the channel has a first cross sectional area. There is also a second portion for restricting the flow of fluid defined by a reproducible deformity in the channel such that the channel has a second cross sectional area which is smaller than the first cross sectional area of the first portion. The tubular member further defines a third portion having a third cross sectional area which is greater than the second cross sectional area of the second portion. All of the cross sectional dimensions of the second and third portions are disposed within a cylindrical envelope having a diameter equal to the diameter of the first portion and extending therefrom. The tubular member is comprised of a material having sufficient rigidity to maintain a preferred reproducible permanent deformity in the channel. Portion has an essentially constant outside diameter and wherein all cross sectional dimensions of the tubular member are less than or equal to the diameter of the first portion and all of the cross sectional dimensions of the second and third portions are disposed within a cylindrical envelope having a diameter equal to the diameter of the first portion and extending therefrom. Preferably, the deformity is defined by a spherical indentation and the tubular member is comprised of stainless steel. The present invention is also a method of forming a fluid restrictor comprising the steps of first placing a tubular member having a channel and a predetermined outside diameter into a fixed relationship with a deforming apparatus. Then, there is the step of deforming the tubular member in a reproducible manner such that a cross sectional area of the channel is decreased so flow of fluid through the channel is restricted a desired amount. The deforming step does not cause the tubular member to flare and all of the cross sectional dimensions of the second and third portions disposed within a cylindrical envelope having a diameter equal to the diameter of the first portion and extending therefrom. Preferably, the deforming step includes the step of forcing a shaping element of the deforming apparatus against the tubular member. Alternatively, the deforming apparatus can be used to adjust the magnitude of fluid restriction during operation of the fluid restrictor.

Fluid restrictor apparatus and method for making the same

A pump for liquefied gases is provided having a low thermal conductivity liner provided in the inner bore of the pump cylinder. The low thermal conductivity liner prevents transmission to the pump cylinder of heat generated during the compression of the liquefied gas. The liner is preferably formed from a ceramic, such as zirconium, or from a plastic. In addition to the provision of the low thermal conductivity liner, techniques for reducing the dead volume of retained gases in the pump chamber are utilized to prevent the transmission of retained heat from the these gases to the incoming charge of liquefied gases.

Liquefied carbon dioxide pump

A thin sample on a surface such as a tissue specimen on a microscope slide is exposed to treatment liquid such as a stain, antibody of nucleic acid probe. The treatment is accelerated by exposing the treatment liquid on the surface to infrared radiation. A device is disclosed for placing such slides into a treatment chamber, for example as a plurality of slide pairs held vertically, with treatment liquid held in capillary gaps between each slide pair. An infrared radiation source provides radiation into the treatment chamber to accelerate the treatment of samples on such slide (on one or both surfaces facing each capillary gap) by the treatment liquid.

Growing community of inventors

Irwin, PA, United States of America

Douglas J Koebler