Pivot Bio, Inc.

Douglas Higgins

Alvin Tamsir

Sarah Bloch

Karsten Temme

Rosemary Clark

Emily Tung

Andrew Abel Prior

Kevin Hammill

Austin Davis-Richardson

The present disclosure is drawn to methods of utilizing nucleic acid barcodes and corresponding amplifying sites in cells in which the barcodes naturally occur. These barcodes and amplifying sites are reconfigured into a single nucleic acid cassette that provides for ease of use in tagging particular species, strains, or variants of cells, each with a different barcode. These barcodes can be used to track the colonization capabilities of the barcoded cells. The present disclosure further provides for assays that utilize natural barcodes to measure relative microbial colonization ability of a plant root system.

Plant colonization assays using natural microbial barcodes

Methods and systems are provided for generating and utilizing a bacterial composition that comprises at least one genetically engineered bacterial strain that fixes atmospheric nitrogen in an agricultural system that has been fertilized with more than 20 lbs of Nitrogen per acre.

Methods and compositions for improving engineered microbes that fix nitrogen

Methods and compositions for improving plant traits

Growing community of inventors

Berkeley, CA, United States of America

Douglas Higgins