Washington University

University of Connecticut

Douglas E Berg

Henry V Huang

Dilip K Nag

Gan Wang

Claire M Berg

A novel transposon useful for sequencing long DNAs is disclosed which comprises a partial sequence of transposon Tn5 with the oligonucleotide primers from phages SP6 and T7 inserted near the opposite ends, respectively, of said transposon Tn5.

DNA transposon Tn5seq1

The present invention discloses a rapid method of restriction mapping, sequencing or localizing genetic features in a segment of deoxyribonucleic acid (DNA) that is up to 42 kb in size. The method in part comprises cloning of the DNA segment in a specialized cloning vector and then isolating nested deletions in either direction in vivo by intramolecular transposition into the cloned DNA. A plasmid has been prepared and disclosed.

Transposon-containing DNA cloning vector and uses thereof

Method of DNA sequencing using DNA transposon Tn5seql

DNA transposon TN5SEQ1

Growing community of inventors

St. Louis, MO, United States of America

Douglas E Berg