Zymogenetics, Inc.

Douglas C Covert

Bruce L Zamost

Chung Chan

Hong Yan Liu

Jeffrey D Meyer

Susan D Holderman

Karen S De Jongh

The expression vectors and methods using anexpression system for the large scale production of IL-21 are described. The vectors utilize the IL-21 coding sequence with specific changes in nucleotides in order to optimize codons and mRNA secondary structure for translation in. Using the expression vectors, the IL-21 gene was produced into a level of greater than 1 g/L in fed batch fermentation. Also included are OmpT deficientstrains transformed with an IL-21 expression vector.

IL-21 production in prokaryotic hosts

The expression vectors and methods using anexpression system for the large scale production of IL-21 are described. The vectors utilize the IL-21 coding sequence with specific changes in nucleotides in order to optimize codons and mRNA secondary structure for translation inUsing the expression vectors, the IL-21 gene was produced into a level of greater than 1 g/L in fed batch fermentation. Also included are OmpT deficientstrains transformed with an IL-21 expression vector.

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Mukilteo, WA, United States of America

Douglas C Covert