Vanderbilt University

Foundation Medicine, Inc.

Douglas B Johnson

Roman Yelensky

Philip James Stephens

Garrett Michael Frampton

Vincent A Miller

James Xin Sun

David Fabrizio

Lisa J Zimmerman

Daniel C Liebler

Hyoungjoo Lee

Carlos A Morales Betanzos

Justin M Balko

Violeta Sanchez De Delgado

Melinda Sanders

Jeffrey Alan Sosman

Christine Marie Lovly

Methods and compositions for treating cancer such as melanomas are disclosed.

Methods of treating cancer

The present disclosure relates generally to the analysis of immune checkpoint proteins involved in cancer. In particular, the present disclosure provides material and methods for determining abundance ratios of various immune checkpoint proteins (e.g., PD-1, PD-L1, and PD-L2) present in a biospecimen sample based on quantification of peak area using mass spectrometry analysis. The methods disclosed herein provide an alternative platform for diagnosing and treating cancer, especially in cases limited by ineffective antibody recognition.

Immune checkpoint molecular fitness profiling by mass spectrometry

A method of detecting cell membrane expression of an MHC molecule in a subject, a method of treating cancer in a subject with an immunotherapeutic agent, and an MHC complex are provided. The method of detecting cell membrane expression of an MHC molecule includes obtaining a tumor cell sample from a subject, and detecting cell membrane expression of the MHC molecule by contacting the tumor cell sample with an antibody targeting the MHC molecule and detecting binding between the MHC molecule and the antibody. The method of treating cancer in a subject with an immunotherapeutic agent includes detecting cell membrane expression of an MHC molecule in the subject and administering a therapeutically effective amount of the immunotherapeutic agent if the level of cell membrane expression of the MHC molecule exceeds a predetermined standard. The complex includes a tumor cell in complex with an antibody or an antigen-binding portion thereof.

Methods and systems for predicting response to immunotherapies for treatment of cancer

Growing community of inventors

Nashville, TN, United States of America

Douglas B Johnson