Children's Medical Center Corporation

Beth Israel Deaconess Medical Center, Inc.

Flagship Pioneering Innovations V, Inc.

Children's Medical Center Corporation and Beth Israel

Deaconess Medical Center, Inc.

Douglas B Cowan

James D McCully

Pedro J Del Nido

Sidney Levitsky

Geoffrey Von Maltzahn

Jacob Rosenblum Rubens

John Miles Milwid

Michael Travis Mee

Sitaram Emani

Kyle Marvin Trudeau

Kiana Mahdaviani

Jacob Feala

David Chess

Christina A Pacak

Thomas Duignan

Sitaram M Emani

The disclosure relates to compositions comprising isolated mitochondria or combined mitochondrial agents, and methods of treating disorders using such compositions.

Therapeutic use of mitochondria and combined mitochondrial agents

An automated isolation device for isolation of mitochondria includes an incubation station including a holder for a viable mitochondria solution; and a cooling system, controlled by a processor of the device, for cooling the holder. The device includes a processor-controlled transfer system for transferring solution from the holder to a filtration station; and the filtration station, including a series of filters. The device includes a processor-controlled spectrometry station including a spectrometer positioned to illuminate a cuvette fluidically coupled to an output of the filtration station; and a detector coupled to the processor and positioned on a side of the cuvette opposite the spectrometer. The device includes a processor-controlled transfer system for transferring solution from the spectrometry station to a centrifuge. The centrifuge is processor-controlled and configured to centrifuge the filtrate to separate viable mitochondria from a supernatant.

Automated isolation of viable mitochondria

Therapeutic chondrisome and mitoplast compositions and related methods are described.

Methods and compositions relating to chondrisomes from blood products

Therapeutic chondrisome compositions and related methods are described.

Methods and compositions relating to chondrisomes from cultured cells

Filtration apparatuses, kits, and methods for rapid isolation of intact, viable mitochondria from tissues are described with mitochondria isolated by differential filtration through nylon mesh filters. Mitochondria can be isolated in less than 30 minutes using the filtration apparatuses, kits, and methods described.

Products and methods to isolate mitochondria

Therapeutic use of mitochondria and combined mitochondrial agent

Growing community of inventors

Brighton, MA, United States of America

Douglas B Cowan