Pioneer Hi-Bred International, Inc.

Douglas A Rice

Virginia C Crane

Edmund H Crane, Iii

Jeanne Sandahl

Carl R Simmons

Gary A Sandahl

John T Tossberg

Lingyu Zhang

Jeanne M Sandahl

The present disclosure provides compositions and methods for regulating expression of heterologous nucleotide sequences in a plant. Compositions include a novel nucleotide sequence for a tissue-preferred maize promoter. A method for expressing a heterologous nucleotide sequence in a plant using the promoter sequence disclosed herein is provided. The method comprises stably incorporating into the genome of a plant cell a nucleotide sequence operably linked to the root-preferred promoter of the present invention and regenerating a stably transformed plant that expresses the nucleotide sequence.

Maize promoter active in silks, stalk nodes, roots and leaf sheaths

The invention provides isolated NPR1 nucleic acids and their encoded proteins. The present invention provides methods and compositions relating to altering NPR1 concentration and/or composition of plants. The invention further provides recombinant expression cassettes, host cells, and transgenic plants. Additionally, the present invention provides NPR1 promoter elements capable of initiating constitutive expression in a plant expression constructs comprising them, cells, plants and seeds comprising the constructs, and methods of using them to express heterologous nucleic acids in a plant. Further, the present invention provides for methods for screening putative activators of a plant resistance pathway.

Maize NPR1 promoter and methods of use in plant gene expression

The invention provides isolated NPR1 nucleic acids and their encoded proteins. The present invention provides methods and compositions relating to altering NPR1 concentration and/or composition of plants. The invention further provides recombinant expression cassettes, host cells, and transgenic plants. Additionally, the present invention provides promoter elements capable of initiating constitutive expression in a plant. Further, the present invention provides for methods for screening putative activators of a plant resistance pathway.

Maize NPR1 polynucleotides and methods of use

The present invention provides compositions and methods for regulating expression of heterologous nucleotide sequences in a plant. Compositions are novel nucleotide sequences for constitutive promoters isolated from maize genes encoding histone H2B, metallothionein, alpha-tubulin 3, elongation factor efla, ribosomal protein rps8, chlorophyll a/b binding protein, and glyceraldehyde-3-phosphate dehydrogenase. A method for constitutively expressing a heterologous nucleotide sequence in a plant using the promoter sequences disclosed herein is provided. The method comprises transforming a plant cell to comprise a heterologous nucleotide sequence operably linked to one of the constitutive promoters of the present invention and regenerating a stably transformed plant from the transformed plant cell.

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Leawood, KS, United States of America

Douglas A Rice