Boehringer Mannheim Gmbh

Dorothea Ambrosius

Rainer Rudolph

Carola Dony

The present invention concerns a process for the isolation of recombinant IgA protease from inclusion bodies. In addition a recombinant DNA is claimed which codes for an IgA protease whose C-terminal helper sequence and preferably also its N-terminal signal sequence is no longer active.

Methods for making neisseria or hemophilus IgA protease and DNA encoding

Process for the reactivation of denatured protein

A process for the activation of recombinant proteins which are present in at least a partially inactive form in which a protein is activated by known solubilization or/and renaturation techniques, said protein having additional helper sequences 2 to 50 amino acids in length at its N- or/and C-terminus whereby the relative hydrophobicity of these helper sequences which is calculated as the sum of the relative hydrophobicity specified in Table 1 for the individual amino acids has a negative numerical value.

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Iffeldorf, Germany

Dorothea Ambrosius