Momenta Pharmaceuticals, Inc.

Dorota A Bulik

Carlos J Bosques

Brian Edward Collins

James Meador, Iii

Nathaniel J Washburn

Naveen Bhatnagar

Ian Christopher Parsons

Lakshmanan Thiruneelakantapillai

Lynn Markowitz

Sathya Prabbhakar

Julia Brown

Sean Smith

Maurice Gaston Hains

Zachary Shriver

Ganesh Venkataraman

James R Myette

Xiangping Zhu

Rajeev Chillakuru

Jay Duffner

James Iii Meador

Brian E Collins

Zachary Schriver

Sulfated glycoproteins, and methods of making and using such sulfated glycoproteins, are described.

Modified glycoproteins

Modified glycoproteins, and methods of making and using such modified glycoproteins, are described.

Methods and compositions related to high mannose glycans are described.

High mannose glycans

The present disclosure provides methods for analyzing structure and/or composition of N-glycans. Such methods often involve digestion of N-glycans with multiple exoglycosidases. In some embodiments, N-glycans are digested with multiple exoglycosidases simultaneously. In some embodiments, N-glycans are digested with multiple exoglycosidases sequentially. In some embodiments, methods in accordance with the present disclosure involve comparison of cleavage products of N-glycans that have been digested with multiple exoglycosidases simultaneously to N-glycans that have been digested with multiple exoglycosidases sequentially.

Characterization of N-glycans using exoglycosidases

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The present disclosure provides methods for assessing the glycosylation of a target glycoprotein produced by a cell through analysis of cell-surface glycans on the cell. The present disclosure therefore teaches that glycosylation of cell surface proteins can serve as a proxy for glycosylation of other proteins.

Methods related to cell surface glycosylation

Growing community of inventors

Malden, MA, United States of America

Dorota A Bulik