Other

Life Technologies Corporation

Invitrogen Corporation

Whatman Gmbh

Donna K Fox

James L Hartley

Michael A Brasch

Gary F Temple

Deb K Chatterjee

Mindy D Goldsborough

A pet carrier garment has a full frontal liner that creates a enclosure within which a small pet can be carried close to the owner's body, thereby providing security, warmth and comfort for the pet, as well as affording ample room for the pet to move about while still keeping the pet securely confined.

Pet carrier garment

The present invention is directed generally to methods facilitating the cloning of nucleic acid molecules. In particular, the invention relates to the use of polymerase inhibitors, including but not limited to anti-polymerase antibodies (such as anti-Taq antibodies) and fragments thereof, to inactivate residual polymerase activity remaining after the amplification (particularly via PCR) of a target nucleic acid molecule. The invention further provides compositions, particularly storage-stable compositions, comprising one or more components, such as one or more restriction endonucleases and one or more polymerase inhibitors, that are useful in cloning amplified or synthesized nucleic acid molecules by the above-described methods. The invention also relates to nucleic acid molecules produced by these methods, and to genetic constructs (such as vectors) and host cells comprising these nucleic acid molecules.

Methods for cloning amplified nucleic acid molecules

Recombinational cloning is provided by the use of nucleic acids, vectors and methods, in vitro and in vivo, for moving or exchanging segments of DNA molecules using engineered recombination sites and recombination proteins to provide chimeric DNA molecules that have the desired characteristic(s) and/or DNA segment(s).

Recombinational cloning using nucleic acids having recombination sites

The invention relates to storage of nucleic acid (particularly mRNA) on a solid support and to using such nucleic acid in nucleic acid synthesis or amplification reactions. In a preferred aspect, the invention provides synthesis of cDNA and cDNA libraries.

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Sykesville, MD, United States of America

Donna K Fox