The Liposome Company, Inc.

Celator Pharmaceuticals, Inc.

Transave Limited

Elan Pharmaceuticalstechnologies, Inc.

Donna J Cabral-Lilly

Andrew S Janoff

Paul R Meers

J Craig Franklin

Lawrence D Mayer

Paul Tardi

David K Watkins

Patrick L Ahl

Shaukat Ali

Charles Pak

Yi Zeng

Tong Shangguan

Ravi K Erukulla

Ravi Erukulla

Paul A Harmon

Robert A Reed

Lyophilized liposomal formulations with two or more encapsulated drugs are disclosed. These formulations display superior drug retention profiles and also maintain size distribution following lyophilization and reconstitution.

Method of lyophilizing liposomes

This invention provides a method to prepare liposome-encapsulated bioactive agents such as nucleic acids, comprising complexation of the bioactive agents in reverse micelles prior to forming liposomes, as well as methods of using the liposomes so formed and formulations to deliver nucleic acids to cells.

Encapsulation of bioactive complexes in liposomes

A liposome including a fusogenic liposome, a linking moiety and a targeting moiety. The fusogenic liposome is a lipid bilayer encapsulating contents. The linking moiety is electrostatically bound to the lipid bilayer, and the targeting moiety is covalently bound to the linking moiety. The liposome may also include a stabilizing moiety interposed between the linking and targeting moieties, and covalently bound to both. Alternatively, the stabilizing and targeting moieties may be covalently bound to separate linking moieties, the linking moieties being electrostatically bound to the lipid bilayer.

Modular targeted liposomal delivery system

Peptide-lipid conjugates are incorporated into liposomes so as to selectively destabilize the liposomes in the vicinity of target peptidase-secreting cells, and hence, to deliver the liposomes to the vicinity of the target cells, or directly into the cells. The liposomes can thus be used to treat mammals for diseases, disorders or conditions, e.g., tumors, microbial infection and inflammations, characterized by the occurrence of peptidase-secreting cells.

Peptide-lipid conjugates, liposomes and lipsomal drug delivery

Liposomal peptide-lipid conjugates and delivery using same

Peptide-lipid conjugates

Endotoxin incorporated into liposomes, lipid bilayers or lipid complexes can be detected by combining an aqueous suspension of the liposomes, lipid complexes or lipid bilayers with a suitable detergent. Preferable detergents, e.g., Lubrol-PX.TM. or a polyoxyethylene ether, solubilize the lipid bilayers, liposomes or lipid complexes at acceptable lipid concentrations, forming micelles therefrom which contain lipid bilayer, liposome or lipid complex lipid, detergent and endotoxin, should it be present. The micelles are then assayed for the presence of endotoxin.

Detection of endotoxin levels in liposomes, lipid bilayers and lipid

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Lawrenceville, NJ, United States of America

Donna J Cabral-Lilly