Zymogenetics, Inc.

Pharming Intellectual Property Bv

Pharmaceutical Proteins Limited

Ppl Therapeutics

Donna E Prunkard

Donald C Foster

Ian Garner

Cindy A Sprecher

Ian Robert Cottingham

Michael L Dalrymple

Simon M Temperley

Michael A Dalrymple

Materials and methods for producing fibrinogen in transgenic non-human mammals are disclosed. DNA segments encoding A&#x3b1;, B&#x3b2; and &#x3b3; chains of fibrinogen are introduced into the germ line of a non-human mammal, and the mammal or its female progeny produces milk containing fibrinogen expressed from the introduced DNA segments. Non-human mammalian embryos and transgenic non-human mammals carrying DNA segments encoding heterologous fibrinogen polypeptide chains are also disclosed.

Production of fibrinogen in transgenic animals

Methods for producing protein C in transgenic non-human mammals are disclosed. The protein C is modified at the two-chain cleavage site between the light and heavy chains of protein C from Lys-Arg to R.sub.1 -R.sub.2 -R.sub.3 -R.sub.4 where R.sub.1 through R.sub.4 are individually Arg or Lys. DNA segments encoding modified protein C are introduced into the germ line of a non-human mammals, and the mammal or its female progeny produces milk containing protein C expressed from the introduced DNA segments. The protein C expressed from the introduced DNA segments has anticoagulant activity when activated. Non-human mammalian embryos and transgenic non-human mammals carrying DNA segments encoding heterologous protein C are also disclosed.

Protein C production in non-human transgenic mammals

Materials and methods for producing fibrinogen in transgenic non-human mammals are disclosed. DNA segments encoding A.alpha., B.beta. and .gamma. chains of fibrinogen are introduced into the germ line of a non-human mammal, and the mammal or its female progeny produces milk containing fibrinogen expressed from the introduced DNA segments. Non-human mammalian embryos and transgenic non-human mammals carrying DNA segments encoding heterologous fibrinogen polypeptide chains are also disclosed.

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Seattle, WA, United States of America

Donna E Prunkard