University of Georgia Research Foundation, Inc.

Donna Cassidy-Hanley

Jacek Gaertig

The ciliated protozoanexemplifies a recombinant system for the expression of heterologous nucleic acids, preferably on the plasma membrane surface. Integration of a heterlogous nucleic acid into the &#x3b2;-tubulin gene, BTU1, of a paclitaxel-sensitivemutant that possesses btu1-IK350M &#x3b2;-tubulin allele allows screening for transformants using negative selection, as transformants have restored paclitaxel resistance. Transgenic ciliated protozoa of the invention can serve as live vaccines. For example, transgenicexpressingi-antigen protein on their surface are effective vehicles for vaccination of freshwater fish against infection by

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Freeville, NY, United States of America

Donna Cassidy-Hanley