The Procter Gamble Company

Arkema Inc.

Donn N Rubingh

Bobby Lee Barnett

Philip Frederick Brode, Iii

Chanchal Kumar Ghosh

Paul Elliott Correa

David John Weisgerber

Elizabeth Ellen Sikorski

Eugene Paul Gosselink

Richard Ray Bott

Alan David Willey

Michael E Burns

Richard Timothy Hartshorn

Andre Cesar Baeck

Thomas P Graycar

Edward Eugene Getty

David Neil DiGiulio

Lori Jean Wilson

Philip F Brode

Xue Wang

Keith R Genco

Theodore C Stephens

Chanchal Kamur Ghosh

A stabilized basic aqueous liquid detergent composition is disclosed. The composition comprises hydrogen peroxide, a hydrogen peroxide stabilizer or a mixture of hydrogen peroxide stabilizers, an anionic surfactant or mixture of anionic surfactants, a non-ionic surfactant or mixture of anionic surfactants, and a builder or a mixture of builders. The composition maintains a homogeneous single phase, maintains active oxygen level, and gives a high level of cleaning performance.

Liquid detergent formulation with hydrogen peroxide

The present disclosure relates to subtilisin protease conjugate comprising a protease moiety and one or more addition moieties. Each addition moiety is covalently attached to an epitope protection position of the protease moiety. The protease conjugates have decreased immunogenicity relative to a parent protease. The present disclosure further relates to cleaning and personal care compositions comprising the protease conjugates.

Protease conjugates having sterically protected epitope regions

The present invention relates to variants of serine proteases having decreased immunogenicity relative to their corresponding wild-type proteases. More particularly, the present invention relates to variants having a modified amino acid sequence of a wild-type amino acid sequence, wherein the modified amino acid sequence comprises a deletion and, optionally, a substitution of one or more specifically identified positions corresponding to subtilisin BPN'. The invention further relates to mutant genes encoding such variants and cleaning and personal care compositions comprising such variants.

Serine protease variants having amino acid deletions and substitutions

The present invention relates to subtilisin 309 variants having a modified amino acid sequence of wild-type subtilisin 309 amino acid sequence, the wild-type amino acid sequence comprising a first loop region, a second loop region, a third loop region, a fourth loop region, a fifth loop region and a sixth loop region; wherein the modified amino acid sequence comprises different amino acids than that occurring in wild-type subtilisin 309 (i.e., substitution) at specifically identified positions in one or more of the loop regions whereby the subtilisin 309 variant has decreased adsorption to, and increased hydrolysis of, an insoluble substrate as compared to the wild-type subtilisin 309. The present invention also relates to DNA sequence encoding such subtilisin 309 variants. The present invention also relates to compositions comprising such subtilisin 309 variants for cleaning a variety of surfaces.

Subtilisin 309 variants having decreased adsorption and increased hydrolysis

The present invention relates to variants of subtilisin-like proteases having decreased immunogenicity relative to their corresponding wild-type proteases. The present invention further relates to such variants additionally having one or more amino acid substitutions in one or more epitope regions or additionally having one or more stabilizing substitutions. The invention further relates to mutant genes encoding such variants and cleaning and personal care compositions comprising such variants.

Subtilisin protease variants having amino acid deletions and substitutions in defined epitope regions

The present invention relates to variants of subtilisin-like proteases having decreased immunogenicity relative to their corresponding wild-type proteases. More particularly, the present invention relates to variants having a modified amino acid sequence of a wild-type amino acid sequence, wherein the modified amino acid sequence comprises a substitution of one or more epitope regions. The invention further relates to mutant genes encoding such variants and cleaning and personal care compositions comprising such variants.

Subtilisin protease variants having amino acid substitutions in defined epitope regions

The present invention relates to variants of serine proteases having decreased immunogenicity relative to their corresponding wild-type proteases. More particularly, the present invention relates to variants having a modified amino acid sequence of a wild-type amino acid sequence, wherein the modified amino acid sequence comprises a substitution of one or more specifically identified positions corresponding to subtilisin BPN&prime;. The invention further relates to mutant genes encoding such variants and cleaning and personal care compositions comprising such variants.

Serine protease variants having amino acid substitutions

The present disclosure relates to subtilisin protease conjugate comprising a protease moiety and one or more addition moieties. Each addition moiety is covalently attached to a clip site protection position of the protease moiety, wherein the clip site protection positions are selected from 13, 14, 15, 16, 18, 19, 20, 21, 84, 85, 88, 158, 159, 160, 161, 162, 163, 164, 165, 170, 186, 191, 192, 193, 194, 196, 259, 260, 261, 262, and 274 corresponding to subtilisin BPN&prime;. The protease conjugates have decreased immunogenicity relative to a parent protease. The present disclosure further relates to cleaning and personal care compositions comprising the protease conjugates.

Protease conjugates having sterically protected clip sites

Protease conjugates such as subtilisin conjugates are provided comprising a protease moiety and one or more addition moieties wherein the protease moiety has a modified amino acid sequence of a parent amino acid sequence. The parent amino acid sequence comprises a first epitope region, a second epitope region and a third epitope region, and the modified amino acid sequence comprises a substitution by a substituting amino acid at one or more positions in one or more of the epitope regions. When a substitution occurs in the first epitope region, the substitution occurs at one or more positions corresponding to positions 70-84 of subtilisin BPN&prime;. When a substitution occurs in the second epitope region, the substitution occurs at one or more positions corresponding to positions 103-126 of subtilisin BPN&prime;. When a substitution occurs in the third epitope region, the substitution occurs at one or more positions corresponding to positions 217-252 of subtilisin BPN&prime;. Each addition moiety is covalently attached to one of the substituting amino acids. Cleaning and personal care compositions are provided containing the protease conjugates which have reduced immunogenicity relative to their corresponding parent proteases.

Proteases having modified amino acid sequences conjugated to addition moieties

The present invention relates to Subtilisin DY variants having a modified amino acid sequence of wild-type Subtilisin DY amino acid sequence, the wild-type amino acid sequence comprising a first loop region, a second loop region, a third loop region, a fourth loop region, a fifth loop region and a sixth loop region; wherein the modified amino acid sequence comprises different amino acids than that occurring in wild-type Subtilisin DY (i.e., substitution) at specifically identified positions in one or more of the loop regions whereby the Subtilisin DY variant has decreased adsorption to, and increased hydrolysis of, an insoluble substrate as compared to the wild-type Subtilisin DY. The present invention also relates to DNA sequences encoding such Subtilisin DY variants. The present invention also relates to compositions comprising such Subtilisin DY variants for cleaning a variety of surfaces.

Subtilisin DY variants having decreased adsorption and increased hydrolysis

The present invention relates to subtilisin Carlsberg variants having a modified amino acid sequence of wild-type subtilisin Carlsberg amino acid sequence, the wild-type amino acid sequence comprising a first loop region, a second loop region, a third loop region, a fourth loop region, a fifth loop region and a sixth loop region; wherein the modified amino acid sequence comprises different amino acids than that occurring in wild-type subtilisin Carlsberg (i.e., substitution) at specifically identified positions in one or more of the loop regions whereby the subtilisin Carlsberg variant has decreased adsorption to, and increased hydrolysis of, an insoluble substrate as compared to the wild-type subtilisin Carlsberg. The present invention also relates to the genes encoding such subtilisin Carlsberg variants. The present invention also relates to compositions comprising such subtilisin Carlsberg variants for cleaning a variety of surfaces.

Subtilisin Carlsberg variants having decreased adsorption and increased hydrolysis

The present invention relates to Proteinase K variants having a modified amino acid sequence of wild-type Proteinase K amino acid sequence, the wild-type amino acid sequence comprising a first loop region, a second loop region, a third loop region, a fourth loop region, a fifth loop region and a sixth loop region; wherein the modified amino acid sequence comprises different amino acids than that occurring in wild-type Proteinase K (i.e., substitution) at specifically identified positions in one or more of the loop regions whereby the Proteinase K variant has decreased adsorption to, and increased hydrolysis of, an insoluble substrate as compared to the wild-type Proteinase K. The present invention also relates to DNA sequences encoding such Proteinase K variants. The present invention also relates to compositions comprising such Proteinase K variants for cleaning a variety of surfaces.

Proteinase K variants having decreased adsorption and increased hydrolysis

The present invention relates to subtilisin BPN&prime; variants comprising at least one or more amino acid positions having a different amino acid than that occurring in wild-type subtilisin BPN&prime; (i.e., substitution) at specifically identified positions, whereby the BPN&prime; variant has decreased adsorption to, and increased hydrolysis of, an insoluble substrate as compared to wild-type subtilisin BPN&prime;. The present invention further relates to various cleaning compositions comprising such BPN&prime; variants.

BPN&prime; variants having decreased adsorption and increased hydrolysis

The present invention relates to subtilisin BPN&prime; variants having a modified amino acid sequence of wild-type BPN&prime; amino acid sequence, the wild-type amino acid sequence comprising a first loop region, a second loop region, a third loop region, a fourth loop region and a fifth loop region; wherein the modified amino acid sequence comprises different amino acids than that occurring in wild-type subtilisin BPN&prime; (i.e., substitution) at specifically identified positions in one or more of the loop regions whereby the BPN&prime; variant has decreased adsorption to, and increased hydrolysis of, an insoluble substrate as compared to the wild-type subtilisin BPN&prime;. The present invention also relates to the genes encoding such subtilisin BPN&prime; variants. The present invention also relates to compositions comprising such subtilisin BPN&prime; variants for cleaning a variety of surfaces.

Growing community of inventors

Cincinnati, OH, United States of America

Donn N Rubingh