The South China University of Technology

Dongming Lan

Bo Yang

Yonghua Wang

Weifei Wang

Daoming Li

Xuehui Li

Zhigang Li

Huayong Chen

Dongqi Zhu

Nan Liu

Fanghua Wang

An enzymatic deacidification method for partial glyceride lipase and PUFA-rich oil, comprising the following steps: 1) mixing a polyunsaturated fatty acid (PUFA)-rich oil with a non-polar organic solvent and a short-chain monohydric alcohol, adding an immobilized partial glyceride lipase to carry out an esterification reaction, wherein the partial glyceride lipase is a mutant obtained by mutating the Phe at the 278th position of Lipase SMG1 as Asn; 2) recovering the immobilized enzyme, and recovering the organic solvent and the monohydric alcohol so as to obtain a deacidified PUFA-rich oil. The partial glyceride lipase does not catalyze alcoholysis of triglyceride and like side reactions, has high deacidification efficiency, low reaction temperature, prevents high temperature oxidation of PUFAs, and the immobilized enzyme may be recovered and reused repeatedly, and thus the present invention has good application prospects in industry.

Method for enzymatic deacidification of polyunsaturated fatty acid-rich oil

An enzymatic method for preparing glyceryl butyrate, comprising: (1) carrying out an esterification reaction between n-butyric acid and glycerol using lipase as a catalyst, ethyl acetate and/or ethyl formate as an additive; (2) separating the reaction product, recovering the additive by distillation under reduced pressure to obtain glyceryl butyrate. The invention uses lipase as a catalyst to catalyze the esterification reaction of n-butyric acid and glycerin at normal temperature and normal pressure, reduces the energy consumption of the reaction. Also, the reaction condition is mild, no side reaction happens, and no water-carrying agent is used. In addition, the additive increases the catalytic efficiency of the lipase and the conversion rate of butyric acid.

Enzymatic method for preparing glyceryl butyrate

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Guangzhou, China

Dongming Lan