Nanjing Drum Tower Hospital

Dongjin Wang

Can Xu

The present invention discloses a staged culture method for in-vitro expansion of human CIK cells and use thereof. In order to solve the problem of increase of a proportion of necrotic cells in CIK cells caused by adding exogenous IL-12 in the prior art, time of IL-12 induction is shortened by twice induction in stages. A primary induction culture medium containing IL-2, IL-7 and the IL-12 is used for primary induction and a re-induction culture medium containing the IL-2, the IL-7 and a CD28 antibody is used for re-induction. The result shows that the proportion of the necrotic cells in the CIK cells obtained by the staged twice induction method is obviously lower than that obtained by a continuous induction culture method commonly used in the prior art. Besides, a necrosis rate is influenced by primary induction time. When the primary induction time is 60 h, the necrosis rate is 1.02% at the lowest, which is reduced by 69.7% compared with that of a control group. The CIK cells obtained by the present application have a low necrosis rate and high activity, and can be widely used in the treatment of tumors.

Staged culture method for in-vitro expansion of human cytokine-induced killer (CIK) and use thereof

A minimally invasive knotter includes a first forcep handle, a second forcep handle, and a clamping head; middle parts of the first forcep handle and the second forcep handle intersect and are connected in a hinged manner, the first forcep handle includes an installation head, and a rotating rod is spherically hinged to the installation head; the clamping head is connected to the rotating rod; the second forcep handle includes a limiting clamp and a moving component; one side of the limiting clamp includes an opening; the limiting clamp is connected to the second forcep handle through the moving component so as to limit the clamping head at a set angle; an end part, backing away from the rotating rod, of the clamping head includes two clamping rods; and the two clamping rods approach to or move away from each other by a power component.

Minimally invasive knotter

Embodiments of the present disclosure provide an efficient bacteriostatic minimally invasive collection device for great saphenous vein, comprising a grip, a trocar cutting knife, and a protective trocar. The protective trocar is disposed inside the trocar cutting knife, the trocar cutting knife is rotationally connected to the protective trocar; the protective trocar passes through the grip; the grip is provided with a drive mechanism for driving the trocar cutting knife to move relative to the grip; a bacteriostatic cylinder is fixedly mounted at one end of the grip, and the trocar cutting knife passes through the bacteriostatic cylinder; an ultraviolet lamp is fixedly mounted inside the bacteriostatic cylinder, and an end of the bacteriostatic cylinder is provided with a cleaning mechanism for cleaning the trocar cutting knife.

Efficient bacteriostatic minimally invasive collection devices for great saphenous vein

A femoral artery cannula for providing lower limb blood perfusion includes an outer tube and an inner tube. The inner tube is fixedly nested inside the outer tube, a lower surface of the outer tube is provided with a plurality of through holes, upper surfaces of outer sides of the outer tube and the inner tube are each provided with a through slot, two ends of lower sides of the two through slots are each fixedly provided with a sliding rod, a sliding plate is connected between the two sliding rods in a movably adjustable manner, a liquid injection tube is fixedly nested in an inner side of the sliding plate, lower sides of the two sliding plates are each provided with a transmission mechanism for pushing the liquid injection tubes to move, and outer sides of the through slots are each provided with a blocking mechanism.

Femoral artery cannula for providing lower limb blood perfusion

A great saphenous vein collection device using a double-sleeve knife, including a grip, a sleeve cutting knife, and a protective sleeve is disclosed. The protective sleeve is provided inside the sleeve cutting knife and the sleeve cutting knife is rotationally connected to the protective sleeve; the protective sleeve penetrates through the grip; the grip is provided with a drive mechanism for driving the sleeve cutting knife to move relative to the grip; a threading cylinder is fixedly mounted at a top of the grip, one end of the protective sleeve is extending into an interior of the threading cylinder, a first fixing mechanism for fixing a guiding wire is provided in the interior of the threading cylinder; a first support rod is fixedly connected to a side wall of the threading cylinder, and a second support rod is fixedly connected to an end of the first support rod; the second support rod is provided with a second fixing mechanism for fixing the guiding wire at an end of the second support rod away from the first support rod.

Great saphenous vein collection devices using double cannula knife

The present invention discloses a left ventricular outflow tract stent and a delivery system. The left ventricular outflow tract stent includes a stent body, the stent body is composed of a plurality of supporting components arranged along a first path coinciding with a left ventricular outflow tract path, each of the supporting components includes an inverted-V-shaped frame and an inverted-trapezoidal frame, and the inverted-V-shaped frames and the inverted-trapezoidal frames are alternately arranged to form peak regions and valley regions; connecting strips are arranged on the supporting components; and the stent delivery system includes a control component, a delivery catheter, an air bag component, a guide component, and a loading component, the air bag component is connected with the control component through the delivery catheter, the guide component is arranged at an end of the air bag component, away from the delivery catheter, and the loading component is arranged at an end of the guide component away from the air bag component. The control component is configured to control the delivery system to accurately carry and release the stent body on the loading component to a proper position of the left ventricular outflow tract, so that the safety of a minimally invasive surgery is improved.

Left ventricular outflow tract stent and delivery system

The present invention relates to the technical field of electrocoagulation cutters, and in particular, to a manual control handheld pressure-adjustable irrigation and suction electrocoagulation cutter, including: a connecting rod, wherein an irrigation channel and a suction channel which are spaced apart from each other are formed in the connecting rod; a cutting head is arranged at a left end of the connecting rod; a suction port communicated to the suction channel is provided on the cutting head; a head end of the irrigation channel penetrates through the left end of the connecting rod; a connecting head is arranged at a right end of the connecting rod; a mechanical conveying mechanism configured to convey tissue debris cut by the electrocoagulation cutter from a tail end of the suction channel to a middle way of the connecting head is arranged on the connecting rod; an irrigation joint and a suction joint are respectively arranged at front and rear ways of the connecting head; a partition block configured to partition the front way of the connecting head from the other two ways is arranged in the connecting head; an adjustment valve communicated to a tail end of the irrigation channel is arranged in the middle of the connecting rod; and a conveying pipe is arranged between the adjustment valve and the front way of the connecting head. The present invention provides an electrocoagulation cutter capable of avoiding pipeline blockage and good cleaning effect.

Manual control handheld pressure-adjustable irrigation and suction electrocoagulation cutter

The present invention discloses a valve compression apparatus, belongs to the technical field of medical apparatus and instruments, and includes a compression shell, a communication tube, a funnel tube, a compression arc plate, and a pressing assembly; the communication tube is smoothly connected to a small-diameter end of the funnel tube; two opposite sides of the compression shell are provided with placement ports respectively, one placement port is communicated with the communication tube, and the other placement port is communicated with the funnel tube; a plurality of operation ports are arranged in a circumferential direction of the funnel tube; the pressing assembly includes a traction plate with a slope, an elastic support assembly, and a moving ring; when the moving ring moves in a direction from the communication tube to the funnel tube, the moving ring simultaneously squeezes a plurality of traction plates, to drive the traction plates to move towards the funnel tube; the compression arc plate is in a one-to-one correspondence with the operation port, and is connected to the traction plate through a connecting rod; when the traction plate moves towards the funnel tube, the compression arc plate passes through the operation port and moves towards an axis of the funnel tube, to quickly compress a valve and avoid damage in the valve compression process.

Valve compression apparatus

A left atrial appendage ligator having a ligature and operating handle. One end of the operating handle is fixedly connected with a support rod, the inner wall of the support rod is fixedly connected with a feeding pipe and locking pipe. A first sliding groove is formed in the inner side wall of the feeding pipe. The inner side wall of the first sliding groove is slidably connected with a first sliding block. The middle part of the first sliding block is fixedly connected with a feeding push rod. One end of the feeding push rod is fixedly connected with a first hook. One side of the first sliding block is fixedly connected with a return spring. The left atrial appendage ligator has a locking push rod, ligature, hanger knot and supporting component. The ligature is prearranged on a sleeve pipe portion to form the hanger knot to avoid knotting during the operation process. During operation, the knotting can be completed only by locking the hanger knot. The sleeve pipe portion serves as support for threading the other end of the ligature to avoid automatic locking. After the sleeve pipe portion is separated from the hanger knot, the hanger knot can be pulled for automatic locking.

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Jiangsu, China

Dongjin Wang