Tianjin University of Science and Technology

Dongguang Xiao

Xuewu Guo

Yefu Chen

Guo Zhang

Peng Zheng

Xiaole Wu

Huan Wang

Ruirui Li

Xinyue Kang

Chunhong Sun

Siyao Huang

Jian Guo

Sen Jiang

Yanfang Chen

Wenqi Shi

Yuanhua Wang

Yanrui Ma

Mengjuan Zhang

Jinying Ren

Yongjing Du

The present disclosure belongs to the field of bioengineering, and relates to breeding of industrial microorganisms, in particular to a genetically engineered strain of, method for constructing the same, and its use for brewing, the genetically engineered strain ofheterogeneously overexpresses an acetaldehyde dehydrogenase gene ALD6, an acetyl-CoA synthase gene ACS1 and an alcohol acyltransferase gene AeAT9. Thestrain with high yield of ethyl acetate and low yield of higher alcohols provided by the present disclosure not only maintains excellent ethanol fermentation characteristics, but also reducing the production of higher alcohols which adversely affect the comfort after drinking, which is of great significance for a well-maintained and strengthened flavor characteristics of Chinese Baijiu, an improved and stabilized quality thereof, and even a reform in the fermentation process thereof.

Genetically engineered strain of, method for constructing the same and its use for brewing

Astrain with high yield of ethyl butyrate and a construction method and an application thereof are provided. The strain is obtained by over-expressing in the starting strain acetyl coenzyme A acyl transferase gene Erg10, 3-hydroxybutyryl coenzyme A dehydrogenase gene Hbd, 3-hydroxybutyryl coenzyme A dehydratase gene Crt, trans-2-enoyl coenzyme A reductase gene Ter, and alcohol acyl transferase gene AAT. Compared to the starting bacteria not producing ethyl butyrate, the yield of ethyl butyrate of the constructed strain reaches 77.33&#xb1;3.79 mg/L, the yield of the ethyl butyrate of the strain with double copy expression of gene Ter and gene AAT reaches 99.65&#xb1;7.32 mg/L, increased by 28.9% compared with the EST strain, and 40.93&#xb1;3.18 mg/L of ethyl crotonate is unexpectedly produced.

strain with high yield of ethyl butyrate and construction method and application ofstrain

Anrecombinant strain with high-yield heavy oil and a construction method and application thereof are provided. Therecombinant strain is obtained by knocking out a pullulan synthetase PUL gene while overexpressing an ACL gene. The obtainedrecombinant strain can significantly increase the yield of heavy oil. After 7-day fermentation with xylose as carbon source, the yield of the heavy oil of the recombinant strain reaches 19.4372 g/L, while the yield of the heavy oil of the original strain is 10.0325 g/L, i.e. the recombinant strain improves the yield by 93.74% compared with the original strain.

Aureobasidium pullulans strains with high-yield heavy oil and construction method and application thereof

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Tianjin, China

Dongguang Xiao