Geneharbor (hk) Technologies Ltd.

Other

Bioright Worldwide Company Limited

Geneharbor (hong Kong) Biotechnologies Limited

Dong Shen

Jun Wang

Rongzhao Fu

Caike Jin

Junming Chen

Liang Sun

Yau Lung Siu

Zhangming Liu

Wing Keung Eric Poon

Junming Room 607 Building No 2 Chen

Zhaoming Unit 506-7 5/F Bio-Informatics Centre Liu

The present invention provides an immobilized reaction device and a method for carrying out reaction by utilizing the immobilization technology. The immobilized reaction device includes a columnar reactor with an inlet and an outlet. The reactor is provided with an interior cavity which is defined by a top portion and a bottom portion opposite to each other, and a side wall connecting the top portion and the bottom portion.

Immobilized reaction device and a method for carrying out a reaction by utilizing the immobilization technology

This invention provides a series of recombinantglucose isomerases with improved catalytic activity and thermostability obtained by using recombinant techniques. These recombinant glucose isomerases comprise amino acid variation including phenylalanine (Phe) at position 139, alanine (Ala) at position 182, serine (Ser) at position 187, and glutamine (Gin) at position 299, and carry at least one additional mutated amino acid at position 87, position 217, position 260 or position 276, and possess a higher catalytic activity than that of the wild-type when using D-glucose as substrate. These recombinant glucose isomerases can be used for direct production of high fructose corn syrup containing 55% [wt] or higher concentration of fructose.

Isolated DNA encoding recombinant glucose isomerase

Methods of using isolated glucose isomerase

This invention describes a series of recombinantglucose isomerases having improved catalytic activity and thermostability. The recombinant glucose isomerases can be used for direct production of fructose syrup containing 55 wt % or higher concentration of fructose, or used for the production of fructose syrup containing less than 55 wt % fructose.

Glucose isomerase mutants

This invention provides a series of recombinantglucose isomerases with improved catalytic activity and thermostability obtained by using recombinant techniques. These recombinant glucose isomerases comprise amino acid variation including phenylalanine (Phe) at position 139, alanine (Ala) at position 182, serine (Ser) at position 187, and glutamine (Gln) at position 299, and carry at least one additional mutated amino acid at position 87, position 217, position 260 or position 276, and possess a higher catalytic activity than that of the wild-type when using D-glucose as substrate. These recombinant glucose isomerases can be used for direct production of high fructose corn syrup containing 55.% [wt] or higher concentration of fructose.

Glucose isomerase mutants, DNA thereof and use thereof

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Hong Kong, China

Dong Shen