Research Corporation Technologies, Inc.

Indiana University

Donald Wiesler

Milos V Novotny

You-Zung Hsieh

Jinping Liu

Osamu Shirota

The present invention is directed to derivatives of aroyl-2-quinoline-carboxaldehyde and their use in detection and quantification of minute amounts of primary amines.

Reagents for detection of primary amines

Quinoline-2-(carboxaldehyde) reagents for detection of primary amines

Compounds containing .alpha.-discarbonyl groups are rendered capable of fluorescent emission by conjugation with carboxylic acid-terminated ortho-diaminoaryl reagents. The carboxylic acid groups serve as ionizable moieties which facilitate the conjugation reaction, and are retained in the conjugation product to enable, or enhance the ability of, the conjugated compounds to be separated in chromatographic and electromigration separation processes, notably electrophoresis. Ortho-dinitro derivatives of the reagents are also disclosed, these derivatives offering the advantage of stability as well as the ability to be used directly in the reaction in the presence of a reducing agent.

Fluorogenic reagents for detection of glycoconjugates, .alpha.-ketoacids

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Bloomington, IN, United States of America

Donald Wiesler