Collaborative Research, Inc.

Genome Therapeutics Corporation

Donald T Moir

Jen-i Mao

Alison Taunton-Rigby

Gerald F Vovis

Bernadette L Alford

Ronald W Davis

Gerald R Fink

David Botstein

Christopher G Goff

Robert G Knowlton

Norton P Peet

John D Williams

Matthew Torhan

Daniel Aiello

Living cells containing genetic material derived from recombinant DNA material and capable of expressing rennin, pre-prorennin and prorennin. The rennin, pre-prorennin and prorennin are derived from cells which are themselves or have had parents thereof treated by recombinant DNA methods to allow production of the desired enzymatic proteins during growth in culture.

Recombinant DNA means and method for producing rennin, prorenin and

Processes for producing pre-prorennin, prorennin and rennin

A DNA segment contains a GAL1 promoter of Saccharomyces cerevisae linked to a gene other than the galoctokinase gene, for directing the expression of the gene within a yeast cell. A GAL1 promoter portion of Saccharomyces cerevisae is linked to a foreign DNA segment for use in expressing a desired protein. Yeast cells containing the GAL1 promoter linked to a foreign DNA segment are grown in a medium containing glucose, wherein the yeast cells metabolize the glucose and are permitted to express a polypeptide when galactose is present in the medium.

Use of the GAL1 yeast promoter

Recombinant DNA means and method

A DNA segment containing a GAL1 promoter linked to a gene other than the galactokinase gene for directing the expression of the gene within a yeast cell.

GAL1 yeast promoter linked to non galactokinase gene

Growing community of inventors

Waltham, MA, United States of America

Donald T Moir