Becton Dickinson and Company Limited

Donald Ray Callihan

Adrien P Malick

John D Mantlo

Patrick Shawn Beaty

Susan M Kircher

William B Brasso

Liping Feng

Ben Turng

Julie L Rosales

Vanda White

Dyan Luper

Curtis M Gosnell

James Y Zhou

Jeffery H Bruton

John P Douglass

Paul Ernest Goldenbaum

William Francis Seip

Various embodiments disclosed herein provide for reagents and methods for rapidly isolating viable microbial cells, including, from positive blood culture samples. The resulting microbial pellet can be used for both identification and growth-based methods such as antimicrobial susceptibility testing. The buffers described herein may contain a base solution, non-ionic detergents, thiols, and optionally, ammonium chloride. The disclosed methods provide a process for rapidly isolating and concentrating viable microorganism(s) from PBC samples using only one sample preparation tube and centrifugation while removing cellular debris from the mammalian blood cells that may interfere with identification methods.

Formulations and process for isolating viable microorganisms from positive blood cultures

Various embodiments disclosed herein provide for reagents and methods for rapidly isolating viable microbial cells, including, from positive blood culture samples. The resulting microbial pellet can be used for both identification and growth-based methods such as antimicrobial susceptibility testing. The buffers described herein may contain a base solution, non-ionic detergents, thiols, and optionally, ammonium chloride. The disclosed methods provide a process for rapidly isolating and concentrating viable microorganism (s) from PBC samples using only one sample preparation tube and centrifugation while removing cellular debris from the mammalian blood cells that may interfere with identification methods.

The present invention relates to a novel container and process, and more specifically, to a rectilinear plastic container whose interior is divided into two or more channels (a multi-channel plate) which is filled with a microbial growth medium and is utilized in a process for enumeration of microorganisms found in clinical or industrial samples. Different microbial growth media may be placed in adjacent channels of the rectilinear plastic container for the process of the present invention. The process of the present invention demonstrates a more efficient and rapid means to identify and enumerate microorganisms in a sample.

Multi-channel plate

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Cockeysville, MD, United States of America

Donald Ray Callihan