Dow Agrosciences LLC

Mycogen Plant Science, Inc.

Other

Pioneer Hi-bred International, Inc.

Wisconsin Alumni Research Foundation

United States of America, As Represented by the Secretary of Agriculture

Mycogen Corporation

Dowelanco

Corteva Agriscience LLC

Doe Agrosciences LLC

Donald J. Merlo

Otto Folkerts

Donald J Merlo

Terry R Wright

Justin Michael Lira

Nicole Arnold

Timothy Denver Hey

Thomas Meade

Aaron Todd Woosley

Terence Anthony Walsh

Lining Guo

Thomas A Rocheleau

Steven Robert Webb

Richard H Baltz

Andrew E Robinson

Scott Bevan

Jan R Turner

Patti J Treadway

Stephanie L Burton

Jayakumar Pon Samuel

Kelley Ann Smith

Clive Waldron

Gaofeng Lin

James K Petell

Jean L Roberts

Kitisri Sukhapinda

M Christine Broughton

Kathryn P Crawford

Krishnamurthy Madduri

Ignacio Mario Larrinua

Baolong Zhu

Michael J Adang

Scott B Bintrim

Elizabeth E Murray

Weiting W Ni

Erika Snodderley

Kenneth Edwin Narva

James A Strickland

Joel Jay Sheets

Sean Michael Russell

William M Ainley

Krishna M Madduri

Avutu Sambi Reddy

Ann Owens Merlo

Mark Allen Thompson

Barry W Schafer

Rod Aaron Herman

Patricia C Apel-Birkhold

Qihua C Cai

Janna Mai Armstrong

David J Bowen

Jerald Coleman Ensign

Richard H Ffrench-Constant

Liu Shen

Gregory L Orr

Todd A Ciche

L Sue Loesch-Fries

Raymond Fatig

Haley Jo Moon

Amanda D Schleper

Ze Sheng Li

Sue Schoonover

Michael B Blackburn

Avutu S Reddy

Arvind Kumar Thirumalaiswamysekhar

James A McSwiggen

Carla Nanette Yerkes

Manju Gupta

Sandeep Kumar

Ning Zhou

Todd P Glancy

Dayakar Reddy Pareddy

Diane M Retallack

Robert A Houtchens

Steven Lee Evans

Andrew F Worden

Jon Christopher Mitchell

Sara Bennett

Scott A Young

Gregory Alan Bradfisch

Nagesh Sardesai

Charles A Mihaliak

Zhifang Gao

Nancy P Jarvis

Dong Liu

Thomas Skokut

Arvind Kumar ThirumalaiswamySekhar

Brent E Edington

Michael G Zwick

Patricia Ann Owens Merlo

Jon C Michell

Nancy J Eagan

Carol Alexandrescu

Pon Samuel Jayakumar

Geoffrey J Letchworth

Barbara A Zilinskas

Gary D Thompson

Sun Yuejin

Tom Meade

The subject invention provides novel plants that are not only resistant to 2,4-D, but also to pyridyloxyacetate herbicides. Heretofore, there was no expectation or suggestion that a plant with both of these advantageous properties could be produced by the introduction of a single gene. The subject invention also includes plants that produce one or more enzymes of the subject invention 'stacked' together with one or more other herbicide resistance genes. The subject invention enables novel combinations of herbicides to be used in new ways. Furthermore, the subject invention provides novel methods of preventing the development of, and controlling, strains of weeds that are resistant to one or more herbicides such as glyphosate. The preferred enzyme and gene for use according to the subject invention are referred to herein as AAD-12 (AryloxyAlkanoate Dioxygenase). This highly novel discovery is the basis of significant herbicide tolerant crop trait and selectable marker opportunities.

Herbicide resistance genes

The subject invention provides novel plants that are not only resistant to 2,4-D and other phenoxy auxin herbicides, but also to aryloxyphenoxypropionate herbicides. Heretofore, there was no expectation or suggestion that a plant with both of these advantageous properties could be produced by the introduction of a single gene. The subject invention also includes plants that produce one or more enzymes of the subject invention alone or 'stacked' together with another herbicide resistance gene, preferably a glyphosate resistance gene, so as to provide broader and more robust weed control, increased treatment flexibility, and improved herbicide resistance management options. More specifically, preferred enzymes and genes for use according to the subject invention are referred to herein as AAD (aryloxyalkanoate dioxygenase) genes and proteins. No &#x3b1;-ketoglutarate-dependent dioxygenase enzyme has previously been reported to have the ability to degrade herbicides of different chemical classes and modes of action. This highly novel discovery is the basis of significant herbicide tolerant crop trait opportunities as well as development of selectable marker technology. The subject invention also includes related methods of controlling weeds. The subject invention enables novel combinations of herbicides to be used in new ways. Furthermore, the subject invention provides novel methods of preventing the formation of, and controlling, weeds that are resistant (or naturally more tolerant) to one or more herbicides such as glyphosate.

This disclosure concerns methods for the design of synthetic nucleic acid sequences that encode polypeptide amino acid repeat regions. This disclosure also concerns the use of such sequences to express a polypeptide of interest that comprises amino acid repeat regions, and organisms comprising such sequences.

Process for designing diverged, codon-optimized large repeated DNA sequences

The subject invention relates to a novel gene referred to herein as DSM-2. This gene was identified inA3. The DSM-2 protein is distantly related to PAT and BAR. The subject invention also provides plant-optimized genes encoding DSM-2 proteins, DSM-2 can be used as a transgenic trait to impart tolerance in plants and plant cells to the herbicides glufosinate and bialaphos. One preferred use of the subject genes are as selectable markers. The use of this gene as a selectable marker in a bacterial system can increase efficiency for plant transformations. Use of DSM-2 as the sole selection marker eliminates the need for an additional medicinal antibiotic marker (such as ampicillin resistance) during cloning. Various other uses are also possible according to the subject invention.

Selectable marker genes

The present disclosure relates to the use of a grass-active herbicide postemergently applied to AAD1-transformed turfgrasses to selectively control grass weeds in a turf grass crop. Also described is the use of AAD1 as a selectable marker in the production of transgenic turfgrass.

Aryloxyphenoxypropionate tolerance in turfrgass species and use of AAD1 as a selectable marker

The present disclosure provides novel compositions and methods for the production and use ofstrains (for example LBA4404) that are deficient in RecA activity relative to the parent strain. Combinations with other gene-deficient-strains ofare also disclosed. Specifically, two exemplary s recA minus strains, UIA777 where chloramphenicol resistant gene disrupting the recA gene and UIA770 where kanamycin resistant gene disrupting the recA gene are provided.

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The subject invention provides novel plants that are not only resistant to 2,4-D, but also to a pyridyloxyacetate herbicide. The subject invention also includes plants that produce one or more enzymes of the subject invention 'stacked' together with one or more other herbicide resistance genes. The subject invention enables novel combinations of herbicides to be used in new ways. Furthermore, the subject invention provides novel methods of preventing the development of, and controlling, strains of weeds that are resistant to one or more herbicides such as glyphosate. The preferred enzyme and gene for use according to the subject invention are referred to herein as AAD-13 (AryloxyAlkanoate Dioxygenase). This highly novel discovery is the basis of significant herbicide tolerant crop trait and selectable marker opportunities.

Uses and detection of herbicide resistance genes for resistance to  aryloxyalkanoate herbicides

strains that harbor transformation-enhancing genes on a plasmid capable of replication independently of thechromosome, the Ti plasmid, and plant transformation binary vectors, and uses for thesestrains are provided. Additionally,strains that are deficient in DNA recombination functions that result in instability or rearrangement of plant transformation binary vectors, and that harbor transformation-enhancing genes on a plasmid capable of replication independently of thechromosome, the Ti plasmid, and plant transformation binary vectors, and uses for these strains, are also provided. Further included arestrains that harbor transformation-enhancing genes integrated into thechromosome at a locus that does not interfere with or otherwise compromise the normal growth and plant transformation ability of thecells, and uses for thesestrains. Plants made using thesestrains are also described.

This invention relates generally to a plant cell with increased yield, preferably under condition of transient and repetitive abiotic stress as compared to a corresponding non-transformed wild type plant cell by increasing or generating one or more activities of RING proteins in plants.

Plants having stress related traits and methods for making the same

The invention provides synthetic nucleic acid sequences encoding proteins of interest that are particularly adapted to express well in plants. The claimed synthetic sequences utilize plant-optimized codons roughly in the same frequency at which they are utilized, on average, in genes naturally occurring in the plant species. The invention further includes synthetic DNA sequence for herbicide tolerance, water and/or heat stress tolerance, healthy oil modifications and for transformation marker genes and selectable marker genes are used. DNA construct and transgenic plants containing the synthetic sequences are taught as are methods and compositions for using the plants in agriculture.

Synthetic genes

Growing community of inventors

Carmel, IN, United States of America

Donald J Merlo