Harvard College

Children's Medical Center Corporation

Massachusetts Institute of Technology

Other

The General Hospital Corporation

Takeda Chemical Industries, Inc.

The Charles Stark Draper Laboratory, Inc.

Depuy Acromed, Inc.

The Brigham and Women's Hospital, Inc.

Vanderbilt University

Tufts University

President and Fellows of Havard College

Wen, Norman

Novak, Richard

Fernandez-Alcon, Jose

Donald Elliot Ingber

Michael Super

Daniel Levner

Christopher David Hinojosa

Alexander L Watters

Mark J Cartwright

George M Whitesides

Geraldine A Hamilton

Jeffrey Charles Way

Jose Fernandez-Alcon

Gregory N Stephanopoulos

Amit Kumar

Gabriel P Lopez

Kevin Kit Parker

Norman Wen

Josiah Daniel Sliz

Josue A Goss

Joanna Aizenberg

Moses J Folkman

Antonio Varone

Takeshi Fujita

Guy Robert Thompson, Ii

Tak Sing Wong

Justin Nguyen

Robert Samuel Langer

Daniel T Chiu

David J Mooney

Jason Oliver Fiering

Emanuele Ostuni

Edward J Doherty

Judah M Folkman

Omar Abdel-Rahman Ali

Tsuneo Kanamaru

Andy H Levine

Jeffrey T Borenstein

Joseph P Vacanti

John P Wikswo

Dennis L Kasper

Shoji Kishimoto

Ronald S Reiserer

Mark Joseph Mescher

Dongeun Huh

Philip LeDuc

Shuichi Takayama

Philseok Kim

Martin M Rottman

Richard Novak

Guy Thompson

Brendon Dusel

Rahul Singhvi

Anthony Bahinski

Sui Huang

Daniel I C Wang

Robert Cunningham

Philip T Snell

Julia B Berthet

Dinah R Super

Benjamin Hatton

Magdalena Kasendra

Remi Villenave

Hyun Jung Kim

Daniel C Leslie

Julie A Tomolonis

Evangelia Murray

Hubert Geisler

Karel Domansky

Youngjae Choe

Anna Waterhouse

Carolina Lucchesi

Daniel I Wang

Mathew Varghese

John V Hamilton

Alexander G Stafford

Janelle R Anderson

Bryan Hassell

Lian Leng

Insung S Choi

Chong Wing Yung

Christopher S Chen

Dmitry A Markov

Dennis Paul Orgill

Riccardo Barrile

David Edward Cliffel

Philip C Samson

Jeffrey J Fredberg

Michael Karpelson

Lisa J McCawley

Andrew L Frelinger, Iii

Noo Li Jeon

Frank Emmanuel Block

Jefferson Puerta

Georg Bauer

James P Butler

Robert M Westervelt

Andries Van Der Meer

Martin Bechthold

John A Mclean

Alfred Paris

Jennifer Robin McKenzie

Jonathan L Grinham

A device for simulating a function of a tissue includes a first structure, a second structure, and a membrane. The first structure defines a first chamber. The first chamber includes a matrix disposed therein and an opened region. The second structure defines a second chamber. The membrane is located at an interface region between the first chamber and the second chamber. The membrane includes a first side facing toward the first chamber and a second side facing toward the second chamber. The membrane separates the first chamber from the second chamber.

Open-top microfluidic devices and methods for simulating a function of a tissue

Described herein are methods for providing an in vitro intestinal model system, e.g., using primary cells instead of cell lines and/or cancerous cells.

Methods relating to intestinal organ-on-a-chip

The invention provides integrated Organ-on-Chip microphysiological systems representations of living Organs and support structures for such microphysiological systems.

Integrated human organ-on-chip microphysiological systems

The present disclosure relates to a microfluidic devices and methods for culturing bone marrow cells. Aspects include methods of preparing microfluidic devices and culturing bone marrow cells with the microfluidic devices. In some aspects, a method includes providing a microfluidic device having an upper chamber, a lower chamber, and a porous membrane separating the upper chamber from the lower chamber. The method further includes seeding walls of the lower chamber and a bottom surface of the membrane with endothelial cells. The method further includes providing a matrix within the upper chamber. The matrix includes fibrin gel and bone marrow cells. The method further includes filling or perfusing the upper chamber with a media.

Bone marrow microfluidic devices and methods for preparing and using the same

An organomimetic device includes a microfluidic device that can be used to culture cells in its microfluidic channels. The organomimetic device can be part of dynamic system that can apply mechanical forces to the cells by modulating the microfluidic device and the flow of fluid through the microfluidic channels. The membrane in the organomimetic device can be modulated mechanically via pneumatic means and/or mechanical means. The organomimetic device can be manufactured by the fabrication of individual components separately, for example, as individual layers that can be subsequently laminated together.

Organomimetic devices and methods of use and manufacturing thereof

Systems and methods interconnect cell culture devices and/or fluidic devices by transferring discrete volumes of fluid between devices. A liquid-handling system collects a volume of fluid from at least one source device and deposits the fluid into at least one destination device. In some embodiments, a liquid-handling robot actuates the movement and operation of a fluid collection device in an automated manner to transfer the fluid between the at least one source device and the at least one destination device. In some cases, the at least one source device and the at least one destination device are cell culture devices. The at least one source device and the at least one destination device may be microfluidic or non-microfluidic devices. In some cases, the cell culture devices may be microfluidic cell culture devices. In further cases, the microfluidic cell culture devices may include organ-chips.

Systems and methods for cell culture device interconnection and fluidic device interconnection

Embodiments of various aspects described herein relate to methods, kits, and cell culture media for generation of podocytes from pluripotent stem (PS) cells, as well as cells produced by the same, and methods of use.

Methods for generation of podocytes from pluripotent stem cells and cells produced by the same

Described herein are heme-binding compositions and methods relating to their use, for example methods of treatment of sepsis and rhabdomyolysis.

Engineered heme-binding compositions and uses thereof

Provided herein relates to systems and methods for producing and using a body having a central channel separated by one or more membranes. The membrane(s) are configured to divide the central channel into at least one mesochannel and at least one microchannel. The height of the mesochannel is substantially greater than the height of the microchannel. A gaseous fluid can be applied through the mesochannel while a liquid fluid flowing through the microchannel. The systems and methods described herein can be used for various applications, including, e.g., growth and differentiation of primary cells such as human lung cells, as well as any other cells requiring low shear and/also stratified structures, or simulation of a microenvironment in living tissues and/or organs (to model physiology or disease states, and/or to identify therapeutic agents and/or vaccines). The systems and methods can also permit co-culture with one or more different cell types.

Low shear microfluidic devices and methods of use and manufacturing thereof

This disclosure provides, e.g., methods for coupling Formation of Surface Carboxylates on PES an entity to a solid substrate. The method can comprise treating the with Exposure Time substrate with a plasma, e.g., a CO2 plasma, to increase its reactivity. The entity can be, e.g., a biological polymer that binds a microbe. Substrates produced by these methods can be used in a variety of applications, including hemodialysis and diagnostic assays.

Aqueous biomolecule coupling on CO2-plasma-activated surfaces

The embodiments of the invention described herein relate to systems and methods for culturing and/or maintaining intestinal cells, tissues and/or organoids in vitro. The cells, tissues and/or organoids cultured according to the methods and systems described herein can mimic or reproduce natural intestinal epithelial structures and behavior as well as support co-culture of intestinal microflora.

Cell culture system

An organ-on-a-chip microfluidic device is disclosed that mimics a human lymph node and/or human lymphoid tissue. The device can include cells from human blood and lymphatic tissue, include an extracellular matrix for the development of immune system components, and provide for the perfusion of fluids and solids resembling blood and lymphatic fluid within micrometer sized channels.

Human lymphoid tissue-on-chip

Growing community of inventors

Boston, MA, United States of America

Donald Elliot Ingber