Amrad Corporation Limited

Donald Bruce Smith

David J Kemp

Nicholas Samaras

Simon J Foote

Michael G Peterson

A recombinant DNA molecule comprising a nucleotide sequence which codes for expression of a fusion protein in which a foreign protein or peptide is fused with the enzyme glutathione-S-transferase, is disclosed, as well as expression vectors or host cells containing such a molecule. Optionally, the foreign protein or peptide is fused to the enzyme through a cleavable link. Also disclosed is an expression vector having inserted therein a nucleotide sequence capable of being expressed as the enzyme glutathione-S-transferase followed by at least one restriction endonuclease recognition site for insertion of a nucleotide sequence capable of being expressed as a foreign protein or peptide fused to the glutathione-S-transferase.

Fusion proteins containing glutathione-s-transferase

A method for capturing amplified target DNA on a solid substrate comprising incorporating a ligand into said DNA by a polymerase chain reaction using a set of primers wherein one of the primers bears the ligand, and contacting the so treated DNA with a solid substrate having a binding reagent for said ligand immobilized thereon.

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Berwickshire, United Kingdom

Donald Bruce Smith