University System of Maryland

Calibrant Biosystems, Inc.

Other

Don DeVoe

Cheng Sheng Lee

Alex Sposito

A microfluidic device including at least one channel in fluid communication with a sample trap array. Specifically, the configuration and geometry of the trap arrays according to the present invention allows for performing sample digitization that supports passive self-discretization within the sample traps without the need for any external flow control or actuation. Geometrical parameters defining the sample traps, including the trap width and the trap depth, are selected to optimize self-filling of the sample traps. Reagents are incorporated into the sample traps during device fabrication to allow for performing multiplexed reactions within the sample traps.

Trap arrays for robust microfluidic sample digitization

The invention provides a microfluidic apparatus for performing 2-D biomolecular separations. The microfluidic 2-D device may include first and second planar substrates which include at least a first dimension microchannel extending in a first direction and an array of second dimension microchannels extending in a second direction, preferably, orthogonal to the first dimension. The ends of at least some of the microchannels are in fluid communication with a plurality of reservoirs. The substrates may further include a number of microchannels and reservoirs. The reservoirs are in electrical communication with a plurality of electrodes and voltage power sources. The device enables two dimensional separations of proteins, DNA and other biomolecules. According to another aspect of the invention, an array of tertiary microchannels extending in a third direction may be utilized.

Two-dimensional microfluidics for protein separations and gene analysis

The invention relates to an apparatus for performing gel protein extractions and methods of using the apparatus.

Microfluidic apparatus for performing gel protein extractions and methods for using the apparatus

The invention provides a microfluidic apparatus for performing 2-D biomolecular separations. The microfluidic 2-D device may include first and second planar substrates which include at least a first dimension microchannel extending in a first direction and an array of second dimension microchannels extending in a second direction, preferably, orthogonal to the first dimension. The ends of at least some of the microchannels are in fluid communication with a plurality of reservoirs. The substrates may further include a number of microchannels and reservoirs. The reservoirs are in electrical communication with a plurality of electrodes and voltage power sources. The device enables two dimensional separations of proteins and other biomolecules. According to another aspect of the invention, an isoelectric point based separation is enabled in a first dimension, and a size based separation in a second dimension.

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Bethesda, MD, United States of America

Don DeVoe