Institut Pasteur

Bio-rad Innovations

Bio-rad Pasteur

Dominique Thierry

Jean-Luc Guesdon

Brigitte Gicquel

Anne Brisson-Noel

Dominique Therese Marie Frechon

Agnes Ullmann

Agn Es Ullmann

Agnes Ullman

Françoise Claudine Lauré

Françoise Claudine Louré

Veronique Vincent

Françoise Claudine Laure

Dominique Thérèse Marie Frechon

Nucleic sequences of plasmid origin are isolated from bacteria of the enterohaemorrhagicgroup (EHEC) and used for the identification of EHEC(s), especially those possessing the genes encoding the virulence factors enterohaemolysin and intimin, and more particularly the specific detection of serotype O157:H7, and in detection kits.

[object Object]

The invention concerns nucleic sequences of plasmid origin, present in bacteria of the group enterohemorrhagic(EHEC), the use of said sequences for searching for EHEC, in particular those having genes coding for enterohemolysin and intimin virulence factors, and more particularly for specific detection of the O157:H7 serotype. The invention also concerns a method using said sequences and detection kits containing them.

Fragments of nucleic acids derived from an appropriate mycobacteria genome, particularly Mycobacterium tuberculosis, their applications in the diagnosis of mycobacteria infections, as well as plasmides containing said fragments. The nucleotidic sequence is comprised of a nucleotidic sequence repeated in the genome of a mycobacterium and specific of the bacillus of tuberculosis and is characterized by a strong hybridation with M. tuberculosis.

Mycobacterial nucleic acid hybridization probes and methods of use

The invention relates to a nucleic acid fragment derived from the Mycobacterium tuberculosis genome, characterized in that it contains one of the sequences I, II, III and IV, defined in the following manner:

Specific detection of the mycobacterium tuberculosis

Nucleotide sequences which hybridize specifically with bacterial strains

Growing community of inventors

Boulogne, France

Dominique Thierry