Adisseo France Sas

Compagnie Generale Des Etablissements Michelin

Alderys

Givaudan S.a.

Dominique Louis

Karine Jaillardon

Dominique Thomas

Vincent Lafaquiere

Odile Ramaen

Mélanie Bremond

Melanie Bremond

Philippe Marliere

Muriel Merkamm

The present invention relates to a living cell, preferably a host cell, that is phloroglucinol resistant. The present invention also relates to a method for producing a phloroglucinol-resistant recombinant host cell. The present invention also relates to a method for producing phloroglucinol.

Phloroglucinol-resistant cell, in particular yeast

The present invention relates to the use of type III polyketide synthases of bacteria, such as actinomycete bacteria, as phloroglucinol synthases. The present invention also relates to the isolated nucleic acid molecules encoding these type III polyketide synthases, and also to the vectors and the host cells comprising such nucleic acid molecules. The present invention also relates to methods for producing phloroglucinol.

Use of type III polyketide synthases from bacteria as phloroglucinol synthases

The present invention relates to the field of bio-production of ectoine. There is a need in the art for ectoine production methods allowing its highly efficient synthesis and secretion. The solution proposed in the present invention is the use of a genetically modified yeast comprising many modifications as described in the present text.

Ectoine-producing yeast

Metabolites produced by a microorganism using oxaloacetate, pyruvate and/or acetyl-CoA as substrate or co-substrate upstream in the biosynthesis pathway, and more particularly using oxaloacetate. There is indeed a need in the art for transformed, in particular recombinant, microorganisms having at least an increased ability to produce oxaloacetate, pyruvate and/or acetyl-CoA, and in particular oxaloacetate, thus allowing an increased capacity to produce metabolites produced using oxaloacetate, pyruvate and/or acetyl-CoA as substrate or co-substrate upstream in the biosynthesis pathway, and in particular amino acids and their derivatives thereof, fatty acids, derivatives from the mevalonate pathway (in particular farnesyl, squalene, lanosterol, cholesterol and derivatives, and dolichols), flavonoides and/or polyketides. The solution proposed is the use of a genetically modified yeast comprising many modifications as described in the present text.

Enhanced metabolite-producing yeast

A method for the bio-production of threonine including genetically modified yeasts and a method in which they are used to produce threonine, as compared to the parent yeasts.

Threonine-producing yeast

Methods for producing phloroglucinol as well as methods of using type III polyketide synthases as phloroglucinol synthases, in particular the type III polyketide synthases of algae, such as eukaryotic ochrophyte algae, are described herein. In addition, polypeptides that have phloroglucinol synthase activity, the isolated nucleic acid molecules encoding these type III polyketide synthases, and the vectors and the host cells comprising such nucleic acid molecules are also described.

Use of type III polyketide synthases as phloroglucinol synthases

Metabolites produced by a microorganism using more particularly oxaloacetate as substrate or co-substrate upstream in the biosynthesis pathway. There is indeed a need in the art for transformed, in particular recombinant, microorganisms having at least an increased ability to produce oxaloacetate, thus allowing an increased capacity to produce oxaloacetate-derived amino acids and amino acid derivatives, the oxaloacetate-derived amino acids and amino acid derivatives being termed oxaloacetate derivatives. The solution is the use of a genetically modified yeast including many modifications as described in the present text.

A method of the bio-production of methionine and/or of its derivatives thereof from a reduced source of sulfur, such as MeSH or MeSNa including genetically modified yeasts, having an increased ability to produce methionine and/or its derivatives thereof, as compared to the parent yeasts.

Methionine-producing yeast

A recombinant yeast having a reduced pyruvate decarboxylase activity, in the g&#xe9;nome of which has been inserted: &#x2014;one or more nucleic acids encoding an acetolactate synthase or ALS, &#x2014;one or more nucleic acids encoding an acetolactate decarboxylase or ALD, &#x2014;one or more nucleic acids encoding a butancdiol dehydrogenase or BDH, and &#x2014;one or more copies of a nucleic acids encoding a NADH oxidase or NOXE.

Microorganism strains for the production of 2.3-butanediol

The present invention relates to a recombinant yeast having a reduced pyruvate decarboxylase activity, in the genome of which has been inserted: &#x2014;one or more nucleic acids encoding an acetolactate synthase or ALS, &#x2014;one or more nucleic acids en coding an acetolactate decarboxylase or ALD, and&#x2014;one or more copies of a nucleic acids encoding a NADH oxidase or NOXE.

Method for producing acetoin

Provided is a method for producing L-methionine in which O-phospho-L-homoserine and methanethiol are enzymatically converted into L-methionine and H3PO4. Such a conversion is achieved by an enzyme called O-phospho-L-homoserine (OHPS) dependent methionine synthase. Also described are O-phospho-L-homoserine (OHPS) dependent methionine synthases, i.e. proteins which are able to enzymatically convert O-phospho-L-homoserine and methanethiol into L-methionine and H3PO4 as well as microorganisms which have been genetically modified so as to be able to produce L-methionine from O-phospho-L-homoserine and methanethiol. Furthermore described are methods to screen for enzymes that catalyze the conversion of O-phospho-L-homoserine and methanethiol into L-methionine and HPO.

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Forges les Bains, France

Dominique Louis