Biomerieux, Inc.

Commissariat &#xc0; L'&#xc9;nergie Atomique Et Aux &#xc9;nergies Alternatives

Biomerieux Sa

Dominique Decaux

Frédéric Pinston

Corine Fulchiron

Bruno Colin

Jean-Philippe Charrier

Laurent Drazek

Jean-Claude Raymond

Cécile Paris

Denis Desseree

Lorène Allano

Guillaume Sutra

Christophe Tachier

Guillaume Boissier

Lorette Lapierre

Léontine Jacolin

Guillaume Perrin

Philippine Barlas

Emilie Bisceglia

Régis Montvernay

Loréne Allano

Lorette Munoz

Emilie Collignon

A method for analysing a biological sample by means of an analysis instrument, the sample including biological agents and being arranged in an analysis receptacle in view of a holographic imaging system, the method including: acquiring a holographic image of the sample, the holographic image associating an intensity value with each pixel, determining, from the image acquired, an image mask which associates an active or inactive state with each pixel in accordance with the intensity values so that an inactive state is associated with pixels which correspond to artefacts caused by elements present in the field of view other than biological agents, determining a value of at least one biomass parameter which represents the quantitative spatial distribution of biological agents in the field of view, using only the pixels of the holographic image having an active state, and supplying the value of the biomass parameter from the analysis results.

Method for analysing a biological sample with artefact masking

The aim of the present invention is a method of sampling all or part of a sample () of biological matter (), which is crude, enriched or cultured through contact with a culture medium (), such as agar, for example in a Petri dish, using a probe () equipped with a terminal end (), said sampling method comprising the steps of cooling the terminal end () of the probe (), sticking all or part of the sample () of biological matter () to be sampled through contact, or by applying a pressure exerted by the terminal end () onto the sample () of biological matter (), and sampling of all or part of the sample () of biological matter () so as to separate the sample () of biological matter () from the culture medium (), a method of depositing into a container () or onto an analysis plate () all or part of a sample () of biological matter () stuck onto a frosted terminal end () of a probe, said depositing method including a step of separating the terminal end () of the probe () from all or part of the sample () of biological matter (), as well as a device (), a kit and an apparatus () implementing these methods.

Method of sampling and/or depositing a sample of biological matter and device implementing such method

The present invention relates to a method of sampling biological material on a reference surface, such as the surface of an agar medium, with the aid of a sampling tool, said method making it possible to steer the displacement of the sampling tool towards the reference surface, from a first position towards a second position, and/or of the reference surface towards the sampling tool, from a first position towards a second position, said second position being a contact position in which the sampling tool and the reference surface are in contact, said method comprising the following steps:

Optical method for controlling the movement of a sampling tool

Method of sampling and/or depositing a sample of biological matter and device implementing such a method

An imaging system for generating images of biological samples having a surface, the system comprising: a sample support for supporting a biological sample in use; a plurality of illumination sources, the plurality of illumination sources being arranged around the sample support and each adapted to illuminate the biological sample, in use, from a different direction; an image capture device for capturing illumination which has impinged on the biological sample to thereby form an image of the sample; wherein at least one of the illumination sources direction is not perpendicular to the surface of the sample.

Imaging method and system for analyzing in-vitro biological samples

A method for defining an isolation area around an object of interest in a cell culture vessel, the method comprising the steps of: &#x2014;obtaining one or more images of the cell culture vessel using one or more of a plurality of illumination sources, each illumination source being capable of illuminating the vessel from a different direction; &#x2014;selecting an image or combination of images for further processing; &#x2014;applying a circular object detection transformation to identify one or more objects of interest being substantially circular objects in the cell culture vessel, which circular objects of interest are representative of isolated colonies in the cell culture vessel and determining the center of an object of interest; &#x2014;applying a binarizing step to obtain a binarized image of the object of interest and other objects, wherein the center of the binarized image corresponds to the center of the object of interest; &#x2014;iteratively forming concentric circles with increasing radius, wherein the concentric circles are centered on the center of the binarized image; &#x2014;identifying coronas, wherein a corona is delimited by two circles having successive radius values; &#x2014;for each corona: determining the presence and the location of any other object located in the corona to determine the presence and the location of other objects; &#x2014;determining a clearance angle defining an angular sector free of other objects around the object of interest to define the isolation area around the object of interest.

Bio-imaging method

A method for enhancing an image of a biological sample, which biological sample has been grown in a vessel on a culture medium.

Bio-imaging method and system

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Chaponost, France

Dominique Decaux