Oxford Nanopore Technologies Limited

Domenico Caprotti

Andrew John Heron

Lakmal Nishantha Jayasinghe

Mark Bruce

Rebecca Victoria Bowen

James Anthony Clarke

Elizabeth Jayne Wallace

Szabolcs Soeroes

Luke McNeill

James White

John Milton

Ruth Moysey

David Alves

David Jackson Stoddart

Ramiz Iqbal Nathani

Richard Muscat

Paul Richard Moody

Christopher James Wright

Clive Gavin Brown

Joseph Hargreaves Lloyd

Mihaela Misca

Jessica Mary May Knott

Antonino Puglisi

Anthony Clarke

Jessica Mary May Johnson

Mark John Bruce

Lakmal Jayasinghe

Luke Mcneill

David Antoni Alves

METHODS AND SYSTEMS FOR CHARACTERIZING ANALYTES USING NANOPORES

The invention relates to modified helicases with reduced unbinding from polynucleotides. The helicases can be used to control the movement of polynucleotides and are particularly useful for sequencing polynucleotides.

Modified helicases

Methods of characterizing an analyte using a detector such as a nanopore and an enzyme are provided. One aspect features methods for characterizing a double-stranded polynucleotide using a detector, e.g., without using a hairpin connecting a template and a complement of the double-stranded polynucleotide. Another aspect features methods for characterizing an analyte using a tag-modified nanopore with increased sensitivity and/or higher throughput. Compositions and systems including, e.g., adaptors for attachment to double-stranded polynucleotides and tag-modified nanopores, which can be used in the methods are also provided.

Method of characterizing a polynucleotide

Methods of characterizing an analyte using a nanopore. One aspect features methods for characterizing a double-stranded polynucleotide using a nanopore, e.g., without using a hairpin connecting a template and a complement of the double-stranded polynucleotide. Another aspect features methods for characterizing an analyte using a tag-modified nanopore with increased sensitivity and/or higher throughput. Compositions and systems including, e.g., adaptors for attachment to double-stranded polynucleotides and tag-modified nanopores, which can be used in the methods are also provided.

Methods and systems for characterizing analytes using nanopores

The invention relates to new methods of controlling the movement of polynucleotides through transmembrane pores. The invention also relates to new methods of characterizing target polynucleotides using helicases.

Method for controlling the movement of a polynucleotide through a transmembrane pore

The invention relates to methods using constructs comprising a helicase and an additional polynucleotide binding moiety. The helicase is attached to the polynucleotide binding moiety and the construct has the ability to control the movement of a polynucleotide. The constructs can be used to control the movement of polynucleotides and are particularly useful for sequencing polynucleotides.

Enzyme construct

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Oxford, United Kingdom

Domenico Caprotti