Korea Institute of Ocean Science & Technology

Korea Ocean Research and Development Institute

Dockyu Kim

Joung Han Yim

Se Jong Han

Ha Ju Park

Tai Kyoung Kim

Il Chan Kim

Hyoung Seok Lee

Il-Chan Kim

Jun Hyuck Lee

Chul Soo Shin

Bon-Hun Koo

Pil-Sung Kang

Heeyong Park

Mi Ra Park

Hari Datta Bhattarai

Sung Gu Lee

Keun Sik Kim

Hye Yeon Koh

Yu-Kyeong Park

Seung Jin Kim

A cold-adapted protease derived fromPAMC 21717 or a recombinant cold-adapted protease obtained by expressing a gene encoding the cold-adapted protease inis described, and more particularly, a crystal of a protease exhibiting activity at low temperatures, a method for crystallizing the protease, a method for preparing the protease, a recombinant microorganism that expresses the protease, a method for preparing the recombinant microorganism, a method for preparing the recombinant protease and the use of the cold-adapted protease. The cold-adapted protease exhibits high activity at low temperatures, and securely maintains its enzymatic activity even in the presence of high pH, various metal ions and surfactants. Thus, it is useful in various industrial applications.

[object Object]

Disclosed is a method of the synthesis of ramalin. It comprises reacting a glutamic acid derivative, which is prepared using alkylchloroformate, with a hydrazine salt compound, which is prepared from hydroxy aniline, whether protected or not. The synthesis method allows ramalin, excellent in antioxidant and anti-inflammatory activity, to be simply synthesized at stable yield even without use of a highly toxic solvent such as DMF. In addition, the method is cost competitive, and provides ramalin at high efficiency, thus enabling the mass production of ramalin.

Method for synthesizing ramalin and ramalin precursor by using glutamic acid derivative and hydroxy aniline or hydroxy aniline having protected hydroxy group

The present invention relates to a method for synthesizing ramalin, and more particularly to a method for synthesizing ramalin, which comprises allowing 2-hydrazinylphenol to react with L-glutamic acid having a protected carboxyl group at C-1 and a protected amino group at C-2, and a method for preventing decomposition of the ramalin. According to the present invention, ramalin having excellent antioxidant and anti-inflammatory activities can be synthesized in high yield, and thus can be produced in large amounts. In addition, ramalin can be stably maintained for a long period of time, and thus can be easily used for industrial purposes.

Method for preparing ramalin

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Incheon, South Korea

Dockyu Kim