Sangamo Biosciences, Inc.

Sangamo Therapeutics, Inc.

Dmitry M Guschin

Fyodor Urnov

Gregory J Cost

Michael C Holmes

David Paschon

Phillip Tam

Disclosed herein are donor molecules comprising single-stranded complementary regions flanking one or more sequences of interest. The donor molecules and/or compositions comprising these molecules can be used in methods for targeted integration of an exogenous sequence into a specified region of interest in the genome of a cell.

Targeted genomic modification with partially single-stranded donor molecules

Nucleases and methods of using these nucleases for alteration of a CFTR gene and generation of cells and animal models.

Methods and compositions for alteration of a cystic fibrosis transmembrane conductance regulator (CFTR) gene

Disclosed herein are methods and compositions for targeted deletion of double-stranded DNA. The compositions include fusion proteins comprising a cleavage domain (or cleavage half-domain) and an engineered zinc finger domain, and polynucleotides encoding same. Methods for targeted deletion include introduction of such fusion proteins, or polynucleotides encoding same, into a cell such that two targeted cleavage events occur. Subsequent cellular repair mechanisms result in deletion of sequences between the two cleavage sites.

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Albany, CA, United States of America

Dmitry M Guschin