Life Technologies Corporation

Roche Sequencing Solutions, Inc.

Life Technologies As

Nautilus Subsidiary, Inc.

Nautilus Biotechnology, Inc.

Roche Molecular Systems, Inc.

Genia Technologies, Inc.

Dmitriy Gremyachinskiy

Steven Menchen

Roman Rozhkov

Lai-Qiang Ying

Imad Naasani

Wolfgang Hinz

Barnett B Rosenblum

Geir Fonnum

Hee Chol Kang

Bruce Branchaud

Nini Kjus

Theo Nikiforov

Joseph A Treadway

Joseph M Beechem

Parag Mallick

Daniel Mazur

Alan Blanchard

Shaheer Khan

Eric Tulsky

Prasanna Krishnan Thwar

Kari N Haley

Luisa Andruzzi

Xinzhan Peng

Andrew Trans

John Matthew Mauro

Alexander J Mastroianni

Jeremy Gray

Marc Glazer

David Light

Christina E Inman

Paul M Kenney

Yufang Wang

Ronald L Cicero

Tommie Lloyd Lincecum

Frank Bergmann

Christoph Seidel

Carl Williams Fuller

Peter Crisalli

Alfred Lui

Yuri Belosludtsev

Howard Reese

Craig Stolarczyk

Rachel Galimidi

Tanya Sokolsky

Sujal M Patel

Stephen P Hendricks

Aruna Ayer

Meng Taing

Jason Myers

Michael Dorwart

Christos Tzitzilonis

Victoria L Singer

Torri Elise Rinker

Hannes Kuchelmeister

Gregory Kapp

Tural Aksel

Elvis Ikwa

Diem Tran

Kristina Giorda

Joshua Simon Klein

Sonal Tonapi

Cassandra Stawicki

Ashwini Bhat

Lars Hillringhaus

Scott Conradson

David Dinauer

Bin Zhao

Jeffrey Rossio

Markus Burns

Julia Robinson

Tommie Lloyd Lincecum, Jr

Jo Aaserud

Astrid Evenroed Molteberg

Grete Modahl

M Talha Gokmen

Paul Kenney

Wofgang Hinz

An affinity reagent, having: (a) a retaining component such as a structured nucleic acid particle; and (b) one or both of (i) one or more label components attached to the retaining component, and (ii) one or more binding components attached to the retaining component.

Affinity reagents having enhanced binding and detection characteristics

The present disclosure relates to relates methods and associated compositions that provide fast, efficient site-selective conjugation of a protein, such as the pore-forming protein α-hemolysin, to a biomolecule, such as a DNA polymerase, and the use of such site-selective protein-biomolecule conjugates in nanopore devices and methods.

Site-specific bio-conjugation methods and compositions useful for nanopore systems

The present disclosure relates to compositions and methods based on polypeptide-tagged nucleotide, and the use of such polypeptide-tagged nucleotides in nanopore devices and methods.

Polypeptide tagged nucleotides and use thereof in nucleic acid sequencing by nanopore detection

A method of conjugating a substrate includes exchanging a counter ion associated with a biomolecule with a lipophilic counter ion to form a biomolecule complex, dispersing the biomolecule complex in a nonaqueous solvent, and coupling the biomolecule complex to a substrate in the presence of the nonaqueous solvent.

Conjugated polymeric particle and method of making same

The methods described herein provide a means of producing an array of spatially separated proteins. The method relies on covalently attaching each protein of the plurality of proteins to a structured nucleic acid particle (SNAP), and attaching the SNAPs to a solid support.

Methods of generating nanoarrays and microarrays

A method of forming a polymer matrix array includes treating a surface within a well of a well array with a surface compound including a surface reactive functional group and a radical-forming distal group; applying an aqueous solution including polymer precursors to the well of the well array; and activating the radical-forming distal group of the surface coupling compound with an initiator and atom transfer radical polymerization (ATRP) catalyst to initiate radical polymerization of the polymer precursors within the well of the well array to form the polymer matrix array.

Matrix arrays and methods for making same

Disclosed herein are conjugates comprising a biomolecule linked to a label that have biological activity and are useful in a wide variety of biological applications. For example, provided herein are polymerase-nanoparticle conjugates including a polymerase linked to a nanoparticle, wherein the conjugate has polymerase activity. Such conjugates can exhibit reduced aggregation and improved stochiometries wherein the average biomolecule:nanoparticle ratio approaches or equals 1:1. Also disclosed herein are improved methods for preparing such conjugates, and methods and systems for using such conjugates in biological applications such as nucleotide incorporation, primer extension and single molecule sequencing.

Conjugates of biomolecules to nanoparticles

The present disclosure relates to compounds comprising a negatively-charged polymer moiety which is capable of entering a nanopore and upon entering a nanopore in the presence of positive ions results in an increased flow of the positive ions through the nanopore. The present disclosure provides methods of preparing the compounds and for their use as nanopore-detectable tags, in particular, for nanopore-based nucleic acid detection and sequencing.

Tagged nucleotides useful for nanopore detection

The present disclosure relates to relates methods and associated compositions that provide fast, efficient site-selective conjugation of a protein, such as the pore-forming protein &#x3b1;-hemolysin, to a biomolecule, such as a DNA polymerase, and the use of such site-selective protein-biomolecule conjugates in nanopore devices and methods.

The present disclosure relates to tagged multi-nucleotide compounds, which comprise a single tag moiety covalently linked to a plurality of nucleoside-5'-oligophosphate moieties. As disclosed herein, these tagged multi-nucleotide compounds have improved characteristics as polymerase substrates and can be used in a range of nucleic acid detection and sequencing methods, including nanopore sequencing-by-synthesis.

Tagged multi-nucleotides useful for nucleic acid sequencing

Accordingly, in some embodiments methods for detecting an analyte or analytes in one or more sample(s) are provided. The methods encompass providing a solid support with an addressable marker and an associated ligand, contacting the solid substrate to a sample, thereby forming a contacted solid support, associating the contacted solid support with a FET array and detecting the electrical properties of the FET array and thereby detecting an analyte or analytes in one or more samples. In other embodiments, the sample encompasses a second addressable marker.

Protein detection using FET

A method of forming a polymer matrix array includes applying an aqueous solution into wells of a well array. The aqueous solution includes polymer precursors. The method further includes applying an immiscible fluid over the well array to isolate the aqueous solution within the wells of the well array and polymerizing the polymer precursors isolated in the wells of the well array to form the polymer matrix array. An apparatus includes a sensor array, a well array corresponding to the sensor array, and an array of polymer matrices disposed in the well array.

Growing community of inventors

San Francisco, CA, United States of America

Dmitriy Gremyachinskiy