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United States of America, as Represented by the Secretary of Agriculture

Washington State University

Dmitri V Mavrodi

David M Weller

Linda S Thomashow

Shannon M Delaney

R James Cook

Zhengyu Huang

Jos M Raaijmakers

Jibin Zhang

Mingming Yang

In exemplary embodiments, the invention provides2-79 strains having pyrrolnitrin biosynthetic genes for biocontrol of soilborne diseases.

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Th invention is directed to nucleic acid sequences which encode polypeptides having PhzO activity, namely, the ability to convert phenazine-1-carboxylic acid to a 2-hydroxylated phenazine, and isolated polypeptides having this activity. The invention is also directed to recombinant nucleic acid molecules, vectors, and host cells including the nucleic acid sequences as well as methods for producing and using the polypeptides, including expression in bacterial or plant cells to inhibit fungal pathogens.

Sequences encoding PhzO and methods

The invention is directed to nucleic acid sequences which encode polypeptides having PhzO activity, namely, the ability to convert phenazine-1-carboxylic acid to a 2-hydroxylated phenazine, and isolated polypeptides having this activity. The invention is also directed to recombinant nucleic acid molecules, vectors, and host cells including the nucleic acid sequences as well as methods for producing and using the polypeptides, including expression in bacterial or plant cells to inhibit fungal pathogens.

Transgenic fluorescent Pseudomonas spp. are described which have a biosynthetic locus which encodes for the production of the antibiotic phenazine-1-carboxylic acid stably introduced into the genome, have a locus which encodes for the production of the antibiotic 2,4-diacetylphloroglucinol, and are effective for control of diseases caused by the soil-borne fungus, Rhizoctonia. Strains are also described which control diseases caused by,or Pythium, in addition to Rhizoctonia, or have the ability to control all three diseases.

Growing community of inventors

Pullman, WA, United States of America

Dmitri V Mavrodi