Codexis, Inc.

Dipnath Baidyaroy

Louis Clark

Jie Yang

Xiyun Zhang

Lisa Marie Newman

David Elgart

Ish Kumar Dhawan

Kripa Rao

Lorand Szabo

Sally Rhiannon Postlethwaite

Attila Andor

Andrew Shaw

Rama Voladri

Onorato Campopiano

John H Grate

Brian R Scott

Christopher Hill

Chengsong Liu

Janos Torok

Amala Chokshi

Oleh Tanchak

Jungjoo Yoon

Nicholas John Agard

Goutami Banerjee

Charlene Ching

Jeanne Bonomo Benoit

The present invention provides improved fungal strains. In some embodiments, the improved fungal strains find use in hydrolyzing cellulosic material to glucose.

Fungal strains

The invention provides recombinant GH61 proteins obtained from, and nucleic acids that encode such proteins. The invention also provides protein fractions isolated fromsupernatant that have GH61 protein activity. These preparations can be used to increase yield of products from reactions in which a cellulose-containing substrate undergoes saccharification by one or more cellulase enzymes, such as endoglucanase, &#x3b2;-glucosidase, or cellobiohydrolase. Combinations of GH61 protein and cellulases can be used to break down cellulosic biomass into fermentable sugars in the production of ethanol.

Use of glycohydrolase 61 protein variants with improved thermostability for processing cellulose

The present invention relates to cellobiohydrolase variants having improved thermostability in comparison to wild-type CBH2a.

Cellobiohydrolase variants

The invention relates to expression of a recombinant C1 &#x3b2;-glucosidase. The invention also provides methods for producing a fermentable sugar from cellobiose by contacting cellobiose with a recombinant host cell comprising a polynucleotide sequence encoding C1 &#x3b2;-glucosidase, operably linked to heterologous promoter, under conditions in which &#x3b2;-glucosidase is expressed and secreted by the cell and the cellobiose is enzymatically converted by said &#x3b2;-glucosidase to glucose. Methods of the invention may be used for conversion of a biomass substrate to a fermentable sugar, and ultimately to ethanol or other biofuel.

Recombinant C1 beta-glucosidase for production of sugars from cellulosic biomass

The invention relates to recombinantly produced &#x3b2;-glucosidase variants with enhanced thermoactivity compared to naturally occurring proteins. The invention also provides methods for producing a variant &#x3b2;-glucosidase polypeptide with improved thermoactivity by identifying performance sensitive positions in a target &#x3b2;-glucosidase polypeptide and substituting the residue at that position with a thermoactivity enhancing residue.

Beta-glucosidase variants

The present invention provides compositions and methods for the expression of recombinant &#x3b2;-glucosidase variants, as well as their use in the production of fermentable sugars from cellulosic biomass.

Polynucleotides encoding beta-glucosidase variants

&#x3b2;-glucosidase variants

The present invention provides fungal xylanase and/or xylosidase enzymes suitable for use in saccharification reactions. The present invention provides xylanase and xylosidase enzymes suitable for use in saccharification reactions. The present application further provides genetically modified fungal organisms that produce xylanase(s) and/or xylosidase(s), as well as enzyme mixtures exhibiting enhanced hydrolysis of cellulosic material to fermentable sugars, enzyme mixtures produced by the genetically modified fungal organisms, and methods for producing fermentable sugars from cellulose using such enzyme mixtures. In some embodiments, the xylanase and xylosidase enzyme(s) areenzymes.

Fungal xylanases and xylosidases

The present invention provides various GH61 protein variants comprising various amino acid substitutions. The GH61 protein variants have an improved ability to synergize with cellulase enzymes, thereby increasing the yield of fermentable sugars obtained by saccharification of biomass. In some embodiments, sugars obtained from saccharification are fermented to produce numerous end-products, including but not limited to alcohol.

GH61 glycoside hydrolase protein variants and cofactors that enhance GH61 activity

The invention relates to expression of a recombinant C1 &#x3b2;-glucosidase. The invention also provides methods for producing a fermentable sugar from cellobiose by contacting celiobiose with a recombinant host cell comprising a polynucleotide sequence encoding C1 &#x3b2;-glucosidase, operably linked to heterologous promoter, under conditions in which &#x3b2;-glucosidase is expressed and secreted by the cell and the cellobiose is enzymatically converted by said &#x3b2;-glucosidase to glucose. Methods of the invention may be used for conversion of a biomass substrate to a fermentable sugar, and ultimately to ethanol or other biofuel.

Polynucleotides encoding recombinant C1 beta-glucosidase for production of sugars from cellulosic biomass

The invention relates to recombinantly produced &#x3b2;-Glucosidase Variants with enhanced thermoactivity compared to naturally occurring proteins. The invention also provides methods for producing a variant &#x3b2;-glucosidase polypeptide with improved thermoactivity by identifying performance sensitive positions in a target &#x3b2;-glucosidase polypeptide and substituting the residue at that position with a thermoactivity enhancing residue.

&#x3b2;-Glucosidase variants

The invention provides variants of theCglT beta-glucosidase that have improve beta-glucosidase activity compared to the wild type enzyme. The invention also provides polynucleotides that encode the variants, as well as methods of producing the variants, enzyme compositions comprising the variants, and methods for using the variants in industrial applications.

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Fremont, CA, United States of America

Dipnath Baidyaroy