Stichting Dienst Landbouwkundig Onderzoek

Plant Research International B.v.

Dionisius Elisabeth Antonius Florack

Hendrik Jan Bosch

Hendrikus Antonius Cornelis Bakker

Gerard Johan Adolph Rouwendal

Hendrikus Antonius Cornelus Bakker

Hendrikus Antonius Cornelius Bakker

The present invention relates to non-mammalian &#x3b2;-1,4-galactosyltransferases that can be used in their wild-type or in modified forms. The invention further relates to transformed plants and plant cells expressing non-mammalian &#x3b2;-1,4-galactosyltransferases and methods to produce glycoproteins with altered and preferably mammalian-type glycosylation. The invention additionally provides nucleic acid molecules and expression vectors of non-mammalian &#x3b2;-1,4-galactosyltransferases.

Mammalian-type glycosylation in plants by expression of a zebrafish glycosyltransferase

The invention relates to the field of glycoprotein processing in transgenic plants used as cost efficient and contamination safe factories for the production of recombinant biopharmaceutical proteins or pharmaceutical compositions comprising these glycoproteins. The invention provides a plant comprising a functional mammalian enzyme providing mammalian GnTIII that is normally not present in plants, said plant additionally comprising at least a second mammalian protein or functional fragment thereof that is normally not present in plants.

GNTIII expression in plants

The invention is directed to methods for optimizing glycan processing in organisms (and in particular, plants) so that a glycoprotein having complex type bi-antennary glycans and thus containing galactose residues on both arms and which are devoid of (or reduce in) xylose and fucose can be obtained. The invention is further directed to said glycoprotein obtained and host system comprising said protein.

Optimizing glycan processing in plants

The present invention relates to non-mammalian &#x3b2;-1,4-galactosyltransferases that can be used in their wild-type or in modified forms. The invention further relates to transformed plants and plant cells expressing non-mammalian &#x3b2;-1,4-galactosyltransferase and methods to produce glycoproteins with altered and preferably mammalian-type glycosylation. The invention additionally provides nucleic acid molecules and expression vectors of non-mammalian &#x3b2;-1,4-galactosyltransferases.

Mammalian-type glycosylation in plants by expression of non-mammalian glycosyltransferases

The invention relates to the field of glycoprotein processing in transgenic plants used as cost efficient and contamination safe factories for the production of recombinant biopharmaceutical proteins or pharmaceutical compositions comprising these glycoproteins. The invention provides a plant comprising a functional mammalian enzyme providing mammalian GnTIII that is normally not present in plants, said plant additionally comprising at least a second mammalian protein or functional fragment thereof that is normally not present in plants. 

GnTIII expression in plants

The invention is directed to methods for optimizing glycan processing in organisms (and in particular, plants) so that a glycoprotein having complex type bi-antennary glycans and thus 5 containing galactose residues on both arms and which are devoid of (or reduce in) xylose and fucose can be obtained. The invention is further directed to said glycoprotein obtained and host system comprising said protein.

Growing community of inventors

Wageningen, Netherlands

Dionisius Elisabeth Antonius Florack