Centro De Ingenieria Genetica Y Biotecnologia

Centro De Ingenieria Genetica Y Biotechnologia

Diogenes Quintana Vazquez

Anabel Alvarez Acosta

Consuelo Nazabal Galvez

Alejandro Miguel Martin Dunn

Maria De Jesus Leal Angulo

Alejandro Miguel Dunn

Luis Emilio Carpio Munoz

Carlos Antonio Cano

Enrique Gerardo Nieto

Carlos Antonio Durate Cano

Luis Emilio Munoz

Carmen Elena Gomez Rodriquez

Carmen Elena Rodriquez

Recardo De La Caridid Siva Rodriguez

Enrique Gerardo Guillen Nieto

Recardo De La Caridid Siva Rodriquez

Maria De Angulo

The present invention related to biotechnology and genetic engineering, particularly the expression of proteins of viral origin in microorganisms through their fusion by applying recombinant DNA technology to bacterial peptides. The present invention provides an effcient process for the expression inof heterlogous proteins as fusion peptides with a view to obtaining them with a high degree of purity, in commercially useful amounts, and in an appropriate form for their inclusion in vaccine preparations. What is essentially used is a stabilizing sequence derived from the first 47 amino acids of the antigen P64k ofB:4:P1.15. In particular, use is made of a recombinant plasmid containing said sequence, under the control of the tryptophane promoter ofand of the terminator of the transcription of the phage T4, including restrictions sites which provide for the cloning in phase of DNA fragments coding for polypeptides of interest.

Monoclonal antibody to the stabilizer peptide of the P64K antigen of Neisseria meningitidis

System for the expression of heterologous antigens as fusion proteins

Growing community of inventors

Pinar del Rio, Cuba

Diogenes Quintana Vazquez