Zonhon Biopharma Institute, Inc

Gensun Institute of Biomedicine Co., Ltd.

Dinglong Wu

Jun Wang

Bruce Yong Ma

Chunlin Xu

Yaofang Wang

Chen Chen

Jing Qiu

Methods for use of a multi-arm polyethylene glycol (PEG) modifier in modification of asparaginase. The described multi-arm PEG modifier enhances the subunit interaction of a multimeric protein to maintain the multimeric protein in a polymerized form, thereby improving the stability of the multimeric protein, maintaining the bioactivity of the multimeric protein, and reducing the probability of exposure of the antigen binding site after depolymerization of the subunits, so as to reduce the immunogenicity.

Use of multi-arm polyethylene glycol modifier and application of multi-arm polyethylene glycol modifier in L-asparaginasum modification

The present invention relates to polyethylene glycol (PEG) modified protein drugs, and a PEGylated tissue kallikrein, a preparation method and use thereof are disclosed. The tissue kallikrein has a sequence as shown in SEQ ID No. 1 or SEQ ID No. 2, and the tissue kallikrein may be natural or recombinant. The PEG has a molecular weight of 20 to 40 kDa, and is conjugated to the N-terminal primary amino of the tissue kallikrein. In addition to the advantages of significantly extended half-life, significantly reduced immunogenicity and stable and uniform structure, the biological activity of the PEGylated KLK1 provided in the present invention is improved to a higher extent, which is more significant in the treatment of cerebral apoplexy and diabetic nephropathy in particular.

Pegylated tissue kallikrein, and preparation method therefor and uses thereof

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Jiangsu, China

Dinglong Wu