Medical Research Council

Ludwig Institute for Cancer Research Limited

Dimitris Kioussis

Franklin Grosveld

Franklin Gerardus Grosveld

Mark David Noble

Parmjit Singh Jat

James Ellis

The invention encompasses a locus control subregion that possesses chromatin opening domain activity, the activity conferring reproducible activation of tissue-specific expression on a linked transgene to a non-physiological level when the transgene is integrated in single copy in the genome of a host cell.

Locus control subregions conferring integration-site independent

The provision of cell lines from virtually any cell type of the animal body is greatly facilitated by transgenic non-human eukaryotic animals of the invention in which at least some cells have (i) a differentiation inhibiting sequence chromosomally incorporated under the control of a non-constitutive promotor and/or (ii) a differentiation inhibiting sequence which is itself conditionally active. Said genes are chromosomally incorporated under the control of a promotor such that expression of said sequence is normally held below an effective level, thus allowing normal cell development. However, cells taken from said animal may be prevented from completing differentiation to a non-dividing state in tissue culture by activating expression of said sequence.

Transgenic mice expressing TSSV40 large T antigen

Vectors for the integration of a gene into the genetic material of a mammalian host cell such that the gene may be expressed by the host cell comprise a promoter and the gene and include a dominant activator sequence capable of eliciting host cell-type restricted, integration site independent, copy number dependent expression of the gene.

Vector for integration site independent gene expression in mammalian

Methods for obtaining production of a gene product in a host cell in

Isolated DNA molecules for integration site independent gene expression

Transgenic mouse cells expressing ts SV40 large T

Methods of obtaining a DNA sequence for integration site independent

Vectors for the integration of a gene into genetic material of a mammalian host cell such that the gene may be expressed by the host cell comprise a promoter and the gene and include a dominant ectivator sequence capable of eliciting host cell-type restricted, integration site independent, copy number dependent expression of the gene.

Isolated DNA molecules for intergration site independent gene expression

Growing community of inventors

London, United Kingdom

Dimitris Kioussis