Institut National De La Sante Et De La Recherche Medicale

Biomerieux, Inc.

Ecole Normale Superieur De Lyon

Dimitri Lavillette

Francois-Loic Cosset

Francois Mallet

Els Verhoeyen

Anais Girard-Gagnepain

Jean-Luc Blond

Olivier Bouton

Alessia Ruggieri

The present invention concerns a pseudotyped viral vector particle for transferring biological material into cells, wherein said vector particle comprises at least:&#x2014;a chimeric envelope glycoprotein which comprises or consists in a fusion of the transmembrane and extracellular domain of a baboon endogenous retrovirus (BaEV) envelope glycoprotein and the cytoplasmic tail domain of a murine leukemia virus (MLV) envelope glycoprotein; or&#x2014;a modified BaEV envelope glycoprotein wherein the cytoplasmic tail domain is devoid of the fusion inhibitory R peptide.

Lentiviral vectors pseudotyped with mutant BaEV glycoproteins

A method for detecting the expression of a polypeptide in cells and for detecting the interaction between a polypeptide and cells, ex vivo or in vitro, wherein the polypeptide is selected from the group consisting of: a peptide comprising the cyt domain of the envelope protein of the human endogenous retrovirus, HERV-W; a peptide comprising amino acids 448-538 of SEQ ID NO: 1; and a peptide comprising a sequence having, for any series of 20 amino acids, at least 80% identity with amino acids 448-538 of SEQ ID NO: 1. Detection is established by the fusogenic power of the polypeptide, which is demonstrated by syncytia formation.

Method for detecting the expression of an envelope protein of a human endogenous retrovirus and uses of a gene coding for said protein

Growing community of inventors

Lyons, France

Dimitri Lavillette