Council of Scientific and Industrial Research

Digambar Vitthal Gokhale

Kulbhushan Balwant Bastawde

Uttam Ramrao Kalkote

Rohini Ramesh Joshi

Ramesh Anna Joshi

Sanjay Narayan Nene

Vithal Venkatrao Jogdand

Thottapillil Ravindranathan

Bhaskar Ganapatrao Gaikwad

Ulka Shrirang Puntambekar

Shamrao Ganapatrao Patil

Prabhakar K Ranjekar

Ravindranathan Thottapillil

Sandeep Raghunath Ghorpade

Shamrao Ganpatrao Patil

Bhaskar Ganpatrao Patil

Nutan Dattatraya Mahadik

Kulbhussan Balwant Bastawade

Jayant Malhar Khire

The present invention provides a process for the preparation of acidic lipase from microbial sources with activity at highly acidic pH, the process comprising growing sp. in a fermentation medium containing carbon and nitrogen sources along with nutrients, separating the fungal biomass and recovering the culture filtrate/broth and separating the lipase enzyme.

Process for the preparation of acidic lipase

Process for the preparation of 4(R)-hydroxy cyclopent-2-en1(S)-acetate

A microbial process for the preparation of D(-)-N-carbamoylphenylglycine from DL-5-phenylhydantoin which comprises culturing the strain pseudomonas sp. having Accession No. NCIM 5070 (ATCC 55940) and deposited in National Collection of Industrial Microorganisms (NCIM), National Chemical Laboratory (NCL) (India), having hydantoinase activity, in a medium for 16-20 hours, separating the cells by centrifugation, treating the solid cells obtained with DL-5-phenylhydantoin in buffer, at a temperature in the range of about 25-30&deg; C. for a period of about 2-6 hours, acidifying the mixture to obtain solid D(-)-N-carbamoylphenylglycine, and separating the product by filtration.

Microbial process for the production of D(-)-N-carbamoylphenylglycine

A microbial process for the preparation of D(-)-N-carbamoylphenylglycine from DL-5-phenylhydantoin which comprises culturing the strain pseudomonas sp. having Accession No. NCIM 5070 (ATCC 55940) and deposited in National Collection of Industrial microorganisms (NCIM), National Chemical laboratory (NCL) (India), having hydantoinase activity, in a medium for 16-20 hours, separating the cells by centrifugation, treating the solid cells obtained with DL-5-phenylhydantoin in buffer, at a temperature in the range of about 25-30.degree. C. for a period of about 2-6 hours, acidifying the mixture to obtain solid D(-)-N-carbamoylphenylglycine, and separating the product by filtration.

The subject invention relates to an improved process for the production of alcohol which obviates the drawbacks and limitations of the hitherto known process, comprises growing a yeast in a conventional growth medium, fermenting the medium containing carbon and nitrogen source along with other conventional nutrient ingredients and fruit supplement in whole or in fractions like husk, pulp, powder of seeds and mixture thereof used at least 0.25% by weight allowing the resultant broth to ferment for at least 15 hours at temperature in the range of 15-37.degree. C.

Process for the production of alcohol

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Pune, India

Digambar Vitthal Gokhale