Illumina Corporation

Lawrence Livermore National Security, LLC

Other

Texas Instruments Corporation

University of California

Illumina France Sarl

Dietrich A Dehlinger

Ali Agah

Tracy Helen Fung

Cheng Frank Zhong

Hod Finkelstein

M Shane Bowen

Klint Aaron Rose

Raymond P Mariella, Jr

Andrew Gerrit Huibers

Boyan Boyanov

Peter W Richards

Craig M Ciesla

Arnaud Rival

Darren Segale

Kevin D Ness

Tarun Kumar Khurana

Satyadev R Patel

Poorya Sabounchi

Karl Albert Fisher

Stanley S Hong

Mark R Pratt

Steve S Chiang

Benjamin D Sullivan

Steven Mark Barnard

Robert M Duboc

Alex Aravanis

Maria Candelaria Rogert Bacigalupo

Cyril Delattre

Hongqin Shi

Arash Jamshidi

Aathavan Karunakaran

Bala Murali Venkatesan

Scott Burroughs

Yan-You Lin

Maxim Shusteff

Christopher G Bailey

Juraj Topolancik

Byoungsok Jung

Terry Tarn

Xiuyu Cai

Emrah Kostem

Douglas A Wajda

Brent A Fisher

Danilo Condello

Alex Nemiroski

Frederick G Erie

Anthony Lam

Bojan Obradovic

Michael J Heller

Jennifer Olivia Foley

Marco A Krumbuegel

Yinghua Sun

Aaron Liu

Fabien Abeille

Hamed Amini

Craig Lee Hetherington

Satayadev R Patel

Thomas J Grobelny

Anthony Sun

Hung Nan Chen

Murali Venkatesan

Pargol Gheissari

Jennifer Wang

Majid Babazadeh

Helen Tracy Fung

A method for reducing sequencing by synthesis cycle time using a microfluidic device is provided. The microfluidic device comprises a flow cell having an inlet port, an outlet port, and a flow channel extending between the inlet port and the outlet port, wherein the flow channel receives an analyte of interest and one or more reagents for analyzing and detecting molecules. To aid in the acceleration of the reactions, the microfluidic device comprises a mixing device to increase the rates of diffusion of the reagents from the fluid bulk to an active surface of the flow cell. The mixing device comprises at least one of an electrothermal mixing device, an active mechanical mixing device, and a vibrational mixing device.

Methods and devices for mixing in a microfluidic system

Biosensor including a device base having a sensor array of light sensors and a guide array of light guides. The light guides have input regions that are configured to receive excitation light and light emissions generated by biological or chemical substances. The light guides extend into the device base toward corresponding light sensors and have a filter material. The device base includes device circuitry electrically coupled to the light sensors and configured to transmit data signals. A passivation layer extends over the device base and forms an array of reaction recesses above the light guides. The biosensor also includes peripheral crosstalk shields that at least partially surround corresponding light guides of the guide array to reduce optical crosstalk between adjacent light sensors.

Biosensors for biological or chemical analysis and methods of manufacturing the same

An example image sensor structure includes an image layer. The image layer includes an array of light detectors disposed therein. A device stack is disposed over the image layer. An array of light guides is disposed in the device stack. Each light guide is associated with at least one light detector of the array of light detectors. A passivation stack is disposed over the device stack. The passivation stack includes a bottom surface in direct contact with a top surface of the light guides. An array of nanowells is disposed in a top layer of the passivation stack. Each nanowell is associated with a light guide of the array of light guides. A crosstalk blocking metal structure is disposed in the passivation stack. The crosstalk blocking metal structure reduces crosstalk within the passivation stack.

Image sensor structure

A system includes: an objective lens; a first light source to feed first illuminating light through the objective lens and into a flowcell (e.g., with a relatively thin film waveguide) to be installed in the system, the first illuminating light to be fed using a first grating on the flowcell; and a first image sensor to capture imaging light using the objective lens, wherein the first grating is positioned outside a field of view of the first image sensor. Dual-surface imaging can be performed. Flowcells with multiple swaths bounded by gratings can be used. An auto-alignment process can be performed.

Illumination for fluorescence imaging using objective lens

A device for base calling is provided. The device includes a receptacle configured to hold a biosensor having a sample surface holding a plurality of clusters during a sequence of sampling events, an array of sensors sensing information from clusters disposed in corresponding pixel areas of the sample surface during the sampling events and generate sequences of pixel signals and a communication port configured to output the sequences of pixel signals. The device also includes a signal processor coupled to the communication port and configured to receive and process at least one pixel signal in the sequences of pixel signals that mixes light gathered from at least two clusters in a corresponding pixel area, and to base call each of the at least two clusters using the at least one pixel signal.

Semiconductor-based biosensors for base calling

Flow cells and corresponding methods are provided. The flow cells may include a support frame with top and back sides, and at least one cavity extending from the top side. The flow cells may include at least one light detection device with an active area disposed within the at least one cavity. The flow cells may include a support material disposed within the at least one cavity between the support frame and the periphery of the at least one light detection device coupling them together. The flow cells may include a lid extending over the at least one light detection device and coupled to the support frame about the periphery of the at least one light detection device. The lid and at least a top surface of the at least one light detection device form a flow channel therebetween.

Flow cells and methods related to same

A Light Detection and Ranging (LIDAR) apparatus includes a detector having a first pixel and a second pixel configured to output respective detection signals responsive to light incident thereon, and receiver optics configured to collect the light over a field of view and direct first and second portions of the light to the first and second pixels, respectively. The first pixel includes one or more time of flight (ToF) sensors, and the second pixel includes one or more image sensors. At least one of the receiver optics or arrangement of the first and second pixels in the detector is configured to correlate the first and second pixels such that depth information indicated by the respective detection signals output from the first pixel is correlated with image information indicated by the respective detection signals output from the second pixel. Related devices and methods of operation are also discussed.

Integrated lidar image-sensor devices and systems and related methods of operation

In one embodiment, a sample surface of a biosensor includes pixel areas and holds a plurality of clusters during a sequence of sampling events such that the clusters are distributed unevenly over the pixel areas. In another embodiment, a biosensor has a sample surface that includes pixel areas and an array of wells overlying the pixel areas, the biosensor including two wells and two clusters per pixel area. The two wells per pixel area include a dominant well and a subordinate well. The dominant well has a larger cross section over the pixel area than the subordinate well. In yet another embodiment, an illumination system is coupled to a biosensor that illuminates the pixel areas with different angles of illumination during a sequence of sampling events, including, for a sampling event, illuminating each of the wells with off-axis illumination to produce asymmetrically illuminated well regions in each of the wells.

Systems and devices for high-throughput sequencing with semiconductor-based detection

A Light Detection and Ranging (LIDAR) apparatus includes one or more optical elements configured to direct incident light in one or more directions, and a detector array including a plurality of detector pixels configured to output detection signals responsive to light provided thereto by the one or more optical elements. The light includes scattered light that is redirected relative to the one or more directions. A circuit is configured to receive the detection signals and generate corrected image data based on the detection signals and an expected spread function for the light. Related devices and methods of operation are also discussed.

Glare mitigation in LIDAR applications

A biosensor for base calling is provided. The biosensor comprises a sampling device, which includes a sample surface that has an array of pixel areas and a solid-state imager that has an array of sensors. Each sensor generates pixel signals in each base calling cycle. Each pixel signal represents light gathered in one base calling cycle from one or more clusters in a corresponding pixel area of the sample surface. The biosensor further comprises a signal processor configured for connection to the sampling device. The signal processor receives and processes the pixel signals from the sensors for base calling in a base calling cycle, and uses the pixel signals from fewer sensors than a number of clusters base called in the base calling cycle. The pixel signals from the fewer sensors include at least one pixel signal representing light gathered from at least two clusters in the corresponding pixel area.

High-throughput sequencing with semiconductor-based detection

A flash LIDAR apparatus includes emitter units configured to emit optical signals over a field of view, and detector pixels configured to output detection signals responsive to light representing the optical signals incident thereon. The detection signals correspond to respective phase offsets relative to a frequency of the optical signals. A circuit is configured to determine component measurements corresponding to the respective phase offsets from the detection signals, and calculate a distance of a target from which the light was reflected based on the detection signals. The distance is corrected for motion of the target based on subsets of the component measurements.

Motion correction based on phase vector components

Growing community of inventors

Dublin, CA, United States of America

Dietrich A Dehlinger