Novo Nordisk A/s

Symphogen A/s

Dietmar Weilguny

Lars Soegaard Nielsen

Finn C Wiberg

Anne Bondgaard Tolstrup

Anette Amstrup Pedersen

Christian Muller

Ruth Buemann Simesen

Steffen Faisst

Jan Eric Jensen

Christian Müller

The invention relates to a method for manufacturing a recombinant polyclonal protein composition, in particular a recombinant polyclonal antibody composition. The method comprises obtaining a collection of cells transfected with a collection of variant nucleic acid sequences, wherein each cell in the collection is transfected with and capable of expressing one member of the collection, which encodes a distinct member of a polyclonal protein. The cells are cultured under suitable conditions for expression of the polyclonal protein, which is obtained from the cells or culture supernatant. The nucleic acid sequence is introduced into the cells by transfection with a collection of vectors. The present method is suitable for manufacturing recombinant polyclonal antibodies for therapeutic uses.

Method for manufacturing a recombinant polyclonal protein

The invention provides nucleic acid molecules and vector constructs comprising scaffold/matrix attachment regions and methods of using such scaffold/matrix attachment regions for the industrial production of recombinant proteins and polypeptides.

Method for making recombinant proteins

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Virum, Denmark

Dietmar Weilguny