Kali-chemie Aktiengesellschaft

Gesellschaft Fur Biotechnologische Forschung Mbh

Gesellschaft Fur Biotechnologische Forschung Gmbh

Solvay Enzymes Gmbh & Co. Kg

Gesellschaft Fuer Biotechnologische Forschung Mbh

Gesellschaft Fuer Bio-technologische Forschung

Dietmar Schomburg

Heinrich Steinmetz

Klaus Gerth

Hans Reichenbach

Norbert Bedorf

Rolf Jansen

Gerhard Hofle

Herbert Irschik

Detlef Wilke

Brigitte Kunze

Roman Vetter

Wolfgang Aehle

Wolfram Trowitzsch-Kienast

Victor Wray

Andre Clippe

Hermann Augustiniak

Antoine Amory

Ingo Muecke

Amory Antoine

Harald Sobek

Enzymes stabilized against the destabilizing effect of ionic surfactants and a method of producing such stabilized enzymes in which DNA sequences which code for the enzymes are modified by directed mutation in defined positions which correlate with defined surface regions of the enzyme, in such a way that the codon in which the mutation is located now codes for an amino acid differing from the original amino acid.

Stabilized enzymes and process for preparing them

Novel, optimized highly alkaline proteases which are suitable for use in detergent formulations are prepared by employing microorganisms transformed by mutated DNA sequences. The mutated sequences are obtained starting from DNA sequences which code for highly alkaline protease usually produced by Bacillus species by altering these DNA sequences in defined positions by directed mutagenesis (point mutation) in such a way that the codon in which the point mutation is located now codes for an amino acid which is more strongly basic than the original amino acid. The result is highly alkaline proteases in which original amino acids have been replaced by more strongly basic amino acids, preferably by the amino acids lysine or arginine. Synthetic oligonucleotides, DNA sequences, vectors and transformed microorganisms which are used for generating and obtaining the optimized highly alkaline protease are also described.

Highly alkaline proteases

The invention relates to cyclic antibiotics of formula ##STR1## wherein the prefix R represents that the substituents on the adjacent carbon atom are in the R configuration; the prefix S represents that the substituents on the adjacent carbon atom are in the S configuration;

Macrocyclic antibodies

The invention relates to cyclic antibiotics of formula ##STR1## wherein the prefix R represents that the substituents on the adjacent carbon atom are in the R configuration; the prefix S represents that the substituents on the adjacent carbon atom are in the S configuration; R.sub.1 is hydrogen or hydroxy and R.sub.2 is hydroxy or .beta.-glucopyranosyloxy, and to the hydrates and salts thereof, especially pharmaceutically acceptable salts. The compounds of formula I are prepared by fermentation using the strain So ce 12 (NCIB 12134) of the species Sorangium celulosum and are effective antiobiotics.

Macrocyclic antibiotics

Growing community of inventors

Brunswick, Germany

Dietmar Schomburg