Hoechst Aktiengesellschaft

Aventis Research & Technologies Gmbh & Co Kg

Adventis Research & Technologies Gmbh & Co. Kg

Dieter Wullbrandt

Carlo Giani

Johannes Meiwes

Reinhardt Rothert

Merten Schlingmann

Reinhold Keller

Manfred Schneider

Siegmund Lang

Andreas Brakemeier

Friedrich Engelhardt

Karl-Heinz Keil

Stefan Mullner

Fritz Wagner

Thomas Kiy

Wolfgang Holla

Klaus-Dieter Vorlop

Johann Mixich

Kurt Laumen

Ulrich Klein

Detlef Breitgoff

Merten Schilingmann

Kerstin Steinke

Andreas Seiffert-St&ouml;ricko

The invention relates to sophoroselipids of formula (I) wherein R represents H, &mdash;CH CH , &mdash;CH CH CH , n is an integer number from 2 to 27, R and R represent, independently from each other, H or a group of formula (II), and R represents H or &mdash;OH. The invention also relates to a method for producing sophoroselipids of formula (I) wherein R , R , R and n have the aforementioned meanings and R represents &mdash;CH , &mdash;CH CH or &mdash;CH CH CH . According to this method, a yeast capable of secreting a sophoroselipid in the form of a lactone in the culture excess, is fermented in a culture medium containing a glycerin. a succinate, a mono-, di- and/or tri-saccharide and a lipid precursor, said sophoroslipid being then isolated from the culture solution. The inventive method is characterised in that the lipid precursor contains one or more 3-alkanols, 4-alkanols or an alkanone with a chain length from 6 to 30 carbon atoms or mixtures of said alkanoistalkanone. Furthermore, according to a variant of the aforementioned method for producing sophoroselipids of formula (I), wherein R , R , R and n have the meanings aforementioned and R represents &mdash;CH , &mdash;CH CH or &mdash;CH CH CH , the culture medium is maintained during fermentation under a reduced oxygen concentration. The new sophoroselipids may be used, e.g., as surfactants, cosmetics, disinfecting agents or pharmaceutical products.

Sophoroselipids, method for their production and use

Preparation of microorganisms comprising omega-3-fatty acid for use as a

The present invention relates to glucose- and sophorose-lipids of the formula I ##STR1## in which R.sup.4 is --OH or a group of the formula II, ##STR2## n is an integer from 2 to 26, R.sup.1 and R.sup.2 are, independently of one another, H or ##STR3## R.sup.3 is H or --OH in the case where R.sup.4 is --OH, and is H or --OH or a group of the formula III, ##STR4## in the case where R.sup.4 is a group of the formula II, to a process for obtaining them by fermentation, and to their use as surfactants.

Glucose- and sophorose-lipids, a process for their preparation and their

Bacterial strains are capable of the formation of rhamnolipids, which they generate in the culture solution. If bacteria of the type Pseudomonas aeruginosa are employed for the fermentation, these microorganisms synthesize rhamnolipids in a concentration of 70-120 g/l of culture solution. The L-rhamnose can be recovered directly from the culture solution by hydrolysis of the rhamnolipids, i.e. without a complicated separation of the cell material and without isolation of the rhamnolipids before hydrolysis.

Pseudomonas aeruginosa and its use in a process for the biotechnological

The invention relates to a process for quantitatively purifying glycolipids, wherein the purification of the glycolipids is effected by acidifying the glycolipid-containing solution to pH .ltoreq.5.0, then heating the batch to 60.degree. C.-130.degree. C., and then cooling the batch down to .ltoreq.50.degree. C. and centrifuging it at .gtoreq.500 g in order to sediment the glycolipid-containing phase.

Process for the quantitative purification of glycolipids

The invention relates to .alpha.-L-rhamnosidase, which catalyzes the cleavage of the bond between terminal rhamnose and the aglycone of rhamnose-containing glycosides, a process for preparing it by biotechnological means, and its use for preparing L(+)-rhamnose (6-deoxy-L-mannose).

.alpha.-l-rhamnosidase for obtaining rhamnose, a process for its

Pseudomonas aeruginosa and its use in a process for the biotechnological production of L-rhamnose Bacterial strains are capable of the formation of rhamnolipids, which they generate in the culture solution. If bacteria of the type Pseudomonas aeruginosa are employed for the fermentation, these microorganisms synthesize rhamnolipids in a concentration of 70-120 g/l of culture solution. The L-rhamnose can be recovered directly from the culture solution by hydrolysis of the rhamnolipids, i.e. without a complicated separation of the cell material and without isolation of the rhamnolipids before hydrolysis.

.alpha.-L-Rhamnosidase for obtaining rhamnose, a process for its

The invention relates to polymer conjugates which are reversibly precipitable from aqueous solution and are composed of a carrier polymer which is soluble in aqueous medium and of a catalytically active compound which is chemically bonded thereto. The invention also relates to a process for the preparation of polymer conjugates of this type, and to the use thereof in homogeneous catalysis, especially biocatalysis.

Reversibly precipitable, water-soluble polymer conjugates

Resolution of the racemates of phosphorus-containing functional acetic acid derivatives can be carried out in high yield and in enantiomeric purity using hydrolases such as esterases and lipases.

Process for the enzymatic preparation of optically active phosphorus

Differentiation of the two enantiotopic groups in prochiral glycerol derivatives gives chiral molecules directly, which in turn are easily converted either into the S-series or into the R-series by selective manipulation of the functional groups.

Chiral synthesis units from prochiral glycerol

A process for reacting substrates with biocatalysts in a two-phase liquid system disposed in a columnar vessel having a top portion with an exit opening and a first filter disposed therein, a bottom portion with an entrance opening and a second filter disposed therein, a feed line connecting the exit and entrance openings, and a pump disposed in the feed line. The process comprises the steps of: stirring the two-phase liquid in the columnar vessel to accelerate sedimentation and minimize accumulation of the biocatalysts in the top portion of the vessel; selectively controlling the temperature of the two-phase liquid within the vessel; drawing, with the pump, a portion of the two-phase liquid through the exit opening and first filter; and passing the drawn-off portion of the two-phase liquid through the feed line and through the entrance opening and second filter into the vessel to enhance miscibility of the individual phases of the two-phase liquid with the vessel.

Process for reacting substrates with biocatalysts in a 2-phase liquid

Optically active enantiomers of alkyl .alpha.-phenoxypropionates, and of derivatives thereof, can be racemized in good yields and without formation of decomposition products with the aid of an alkali metal (C.sub.1 -C.sub.5) alcoholate, an alkali metal hydroquinone, an alkali metal phenolate or an alkali metal hydroxyphenoxypropionate, or derivatives thereof.

Process for racemizing optically active alpha-phenoxypropionic acid and

A porous, bead-like copolymer which can be employed to excellent effect for the fixation of enzymes is obtained by suspension polymerization of monomeric oxiranylalkyl compounds and/or 2-aziridinylalkyl compounds, n-valent crosslinking agents, N-vinylamides, heterocyclic 5-membered ring compounds or 6-membered ring compounds, having a polymerizable olefinic group in each case, and polymerizable quaternary ammonium salts.

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Hofheim, Germany

Dieter Wullbrandt