Roche Diagnostics Operations, Inc.

Indiana University

Roche Diagnostics Gmbh

F. Hoffmann-la Roche Ag

Dieter Roessler

Barbara Upmeier

Toralf Zarnt

Elke Faatz

Christian Scholz

Martin Burri

Peter Muench

Sascha Antoni

Bernhard Eckert

Johannes Polz

Andreas Woeste

Werner Doeppen

Elena Pfaffenrot

Disclosed is an immunoassay method for detecting an analyte such as an antigen or an antibody in an isolated sample suspected to contain the analyte by incubating the sample with a plurality of binding partners, one of which carries a detectable label, wherein a label-specific binding partner is added that does not carry a label but binds to the detectable label. The method is applicable for a large variety of analytes and has proven particularly useful for analyte antibodies of the IgG and IgM class present in samples due to infections by pathogens. Also disclosed is a reagent kit useful for the method comprising at least two analyte-specific binding partners one of which carries a detectable label and a label-specific binding partner that binds to said detectable label but itself does not carry a detectable label.

Method for reduction of interferences in immunoassays

The invention concerns variants of JL7 antigens that are suitable for detecting antibodies against(causing Chagas disease) in an isolated biological sample. These antigens comprise a JL7 specific amino acid sequence, said JL7 specific sequence consisting of two copies of SEQ ID NO. 2, wherein each of said two copies has an amino acid identity of at least 90% to SEQ ID NO.2 and wherein no furtherspecific amino acid sequences are present in said polypeptide. The invention also concerns a composition of polypeptides useful for the detection of antibodies againstthat comprises the above characterized JL7 antigen along with at least one ofpolypeptides 1F8, Cruzipain, KMP-11 and PAR-2. Moreover, it relates to a method for producing JL7 antigen as well as to diagnostic methods for detectingantibodies using the JL7 polypeptide. In addition, the invention concerns a reagent kit comprising said JL7 polypeptides or composition ofpolypeptides.

[object Object]

A test management system is presented. The system comprises an analyzer to perform tests a sample according to a first set of instructions, a manager module connected to the analyzer, and a first order interface connected to the manager module. The manager module directs activity of the analyzer according to a second set of instructions. The first order interface receives an order for a first analytical test and a second analytical test and transmits the order to the manager module. If the order is for the first analytical test, the test manager module forwards the order directly to the analyzer and the sample is analyzed by the analyzer according to the first set of instructions. If the order is for the second analytical test, the manager module handles the order according to the second set of instructions and generates and transmits secondary test requests to the analyzer.

Analytical test management system and method

The present invention concerns a composition of polypeptides suitable for detecting antibodies against() in an isolated biological sample consisting of three polypeptides 1F8, JL7 and Cruzipain. A method of producing a soluble and immunoreactive composition of polypeptides suitable for detecting antibodies againstusing said composition of polypeptides is also part of the invention. Moreover, the invention concerns a method for detecting antibodies specific forin an isolated sample wherein a composition of saidpolypeptides is used as well as a reagent kit comprising said composition ofpolypeptides.

Antigen composition for detecting Chagas disease

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Kirchseeon, Germany

Dieter Roessler