Boehringer Mannheim Gmbh

Roche Diagnostics Operations, Inc.

Roche Diagnostics Gmbh

F. Hoffmann-La Roche Ag

Dieter Mangold

Rolf Lerch

Reiner Schlipfenbacher

Siegfried Noetzel

Thomas Fischer

Gerd Zimmermann

Joachim Steinbiss

Juergen Klepp

Juergen Schaeffler

Brigitte Gambke

Jean-Philippe Bogardi

Christian Klein

Erhard Kopetzki

Wolfgang-Reinhold Knappe

Hans Lange

Josef Maier

Christoph Ritter

Norbert Oranth

Hans-Erich Wilk

Gunter Pappert

Manfred Baier

Helmut Jering

Valerie Winckler-Desprez

Werner Stock

Siegfried Notzel

Ellen Mossner

Jurgen Backhaus

Michael Brauner

Gerd Schweiger

Thomas Kotnig

Jelmut Jering

Ulrich Traeger

Gert DI Schweiger

A method for applying reactive films containing solids to microporous membranes is provided wherein the membrane is firstly moistened and the reactive film containing solids is applied to the membrane while it is still moist. Membranes produced in this manner can contain reactive films having a high proportion of film openers and can be used advantageously in diagnostic elements for detecting constituents and, in particular, large hydrophobic analytes in body fluids.

Membranes and methods for coating membranes

The invention relates to an apparatus and a method for determining an analyte in a fluid. Provided in the device is a stirring zone formed in an optionally microfluidic three-dimensional structure, into which stirring zone a sample of the fluid can be introduced and in which there is a stirring device which can be operated in a contactless manner and is optionally designed as a microstirrer, the stirring device being configured so as to stir the sample of fluid introduced into the stirring zone and being loaded with at least one reagent which reacts with the analyte. The invention also provides a stirring device which can be operated in a contactless manner and is optionally designed as a microstirrer, the stirring device being loaded with the at least one reagent which reacts with the analyte.

Apparatus and method for determining an analyte in a fluid

A method for applying reactive films containing solids to microporous membranes is provided wherein the membrane is firstly moistened and the reactive film containing solids is applied to the membrane while it is still moist. Membranes produced in this manner can contain reactive films having a high proportion of film openers and can be used advantageously in diagnostic elements for detecting constituents and, in particular, large hydrophobic analytes in body fluid.

Methods for applying reactive films containing solids to microporous membranes and membranes produced therefrom

An analytical test element for blood analyses is provided having an application site and a microfluidic channel structure in fluid communication with the application site. The channel structure includes at least first and second analytical channels for receiving first and second portions of a blood sample applied to the application site. The first analytical channel includes a first analytical site to determine the total haemoglobin value (Hb) of the blood sample. The second analytical channel includes a second analytical site to determine a glycohaemoglobin value (HbA1c) of the blood sample.

Analytical test element and method for blood analyses

An analytical test element for blood analyses is provided having at least a substrate body having a channel structure for the flow transport of a blood sample from an application site to at least one analytical site. The channel structure has a dilution channel that can be loaded with the blood sample and is provided with a separation component for retaining corpuscular blood components and has a sample channel which conveys a blood sample aliquot to be diluted and joins the dilution channel at a mixing site.

A peptide according to the present invention is not more than 40 amino acids long and contains a sequence which is at least 6 amino acids long from the amino acid partial sequence between the amino acids 144 and 183 of human .alpha.1-microglobulin or/and a sequence which is at least 6 amino acids long from the amino acid partial sequence between the amino acids 1 and 20 of human .alpha.1-microglobulin. An antibody according to the present invention is capable of specific binding to a peptide according to the present invention as well as to human .alpha.1-microglobulin. In order to determine human .alpha.1-microglobulin in a sample liquid by an immunoassay, the sample liquid is brought into contact simultaneously or sequentially with defined amounts of the components antibody and peptide whereby one of the components is labelled and the determination is carried out by means of this label.

Peptides with a characteristic antigenic determinant of

The present invention provides N- and O-substituted aminophenol derivatives of the general formula ##STR1## wherein R.sup.1, R.sup.2, R.sup.3, G and L are as hereinbefore defined. The present invention also provides intermediates for the preparation of these aminophenol derivatives of general formula (I), as well as the use of the aminophenol derivatives of general formula (I) for the determination of hydrolases, as well as for the preparation of agents for carrying out determinations of hydrolysis.

N- and O-substituted aminophenols, method and use for diagnosis

A carrier matrix of polyvinyl alcohol-coated glass is dissolvably impregnated with reagent. The matrix is manufactured by slurrying glass fibers in an excess of water and polyvinyl alcohol and forming a layer, which is then dried and impregnated with reagent.

Carrier matrix with dissolvably impregnated reagent

The present invention is concerned with the use of 1-arylsemicarbazides of the general formula:

Method and diagnostic agent for enzyme substrate stabilization using

The present invention provides N- and O-substituted aminophenol derivatives of the general formula ##STR1## wherein R.sup.1, R.sup.2, R.sup.3, G and L are as hereinbefore defined. The present invention also provides intermediates for the preparation of these aminophenol derivatives of general formula (I), as well as the use of the aminophenol derivatives of general formula (I) for the determination of hydrolyses, as well as for the preparation of agents for carrying out determinations of hydrolyses.

The present invention provides a process for immobilizing a protein or protein containing substance. The material to be immobilized is aggregated, contacted in a liquid with a hydrophilic solid phase and the solid phase, after contact has taken place, is dried. The present invention is also concerned with the solid phase prepared by this process and with the use thereof for analytical determination.

Process for immobilizing a protein containing substance on a solid phase

The invention teaches a method for determining an analyte in a liquid sample via a heterogeneous assay. The sample is contacted to a carrier material which contains, in addition to one of a labelled analyte analogous substance or a labelled analyte specific substance, a detectable component which does not influence immunological reactions occurring during the course of the assay. A solid phase bound component, which is either an insufficient amount of analyte specific substance or an excess of analyte relative to analyte in the sample is contacted to the sample so as to accomplish formation of solid phase bound complexes. After separation of liquid and solid phase, label is measured in one of these, and the detectable substance is measured in the liquid. Correlation of the values obtained leads to determination of the analyte. Also described is a reagent used in the described method.

Heterogeneous immunoassay process

The invention concerns a method for the detection of an analyte in a sample liquid by an enzyme-immunoassay in which an enzyme-labelled compound is partitioned between a solid and a liquid phase and the amount of enzyme label in the liquid phase outside the solid phase is determined as a measure of the concentration of the analyte. The measurement is carried out in a non-porous molding having the liquid phase contained therein in contact with the solid phase. The method is particularly suitable for carrying out in a cuvette which can be filled via the porous matrix or on a test strip having a space in contact with the solid phase.

Enzyme-immunoassay method for the determination of an analyte

A method for carrying out competitive displacement immunoassays which entails the use of an oligovalent labelled antibody having at least 4 binding sites for the analyte to be determined per label.

Displacement immunoassay utilizing an oligavalent labelled antibody

The present invention provides an immune reactive porous carrier material comprising a porous carrier material with immune complexes precipitated thereon, wherein the porous carrier material is treated with at least one wet-strength agent. The present invention also provides processes for the production of this immune reactive porous carrier material.

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Maxdorf, Germany

Dieter Mangold