Rohm Gmbh

Unifar Kimya Sanayi Ve Ticaret A.s.

Siol, Werner Roehm Gmbh Chemishe Fabrik

Dieter Kraemer

Hermann Plainer

Werner Siol

Klaus Lehmann

Norbert Suetterlin

Gerhard Markert

Roland Hartl

Waldemar Schleier

Cornelia Feil

Hans-Ulrich Petereit

Horst Pennewiss

Reiner Schnee

Erwin Schuster

Helmut Uhlig

Roland Schweder

Dieter Helm

Wolfgang Kleese

Dieter Zwara

Ursula Artmann

Bruno Sproessier

Ernst Goeldner

Ferhat Ilhan

Cornelia Scordialo

Beta-lactam antibiotics are synthesized by reacting an amino-beta-lactam component with a corresponding amino-group-containing acylating side-chain component in the presence of penicillin amidase from,covalently immobilized on support particles. The resulting beta-lactam antibiotic product is solubilized by adding an acid such as sulfuric acid to lower the pH to 1.0 at a temperature in the range of 0&deg; C. to &plus;5&deg; C. The immobilized penicillin amidase is substantially inactivated by the acid. After separating the beta-lactam antibiotic product, the immobilized penicillin amidase is substantially reactivated for reuse in antibiotic synthesis by treatment with a buffer having about a neutral pH. Antibiotics that can be produced include ampicillin, amoxicillin, cephalexin, cefaclor and cefadroxil. Support particles that can be used include particles having a macroporous structure and a particle diameter of 10-1000 &mgr;m, particles having oxirane groups, particles made of a synthetic polymer and inorganic particles such as porous glass particles.

Synthesis of beta-lactam antibiotics with immobilized penicillin amidase

A high surface area system is provided with latex particles for immobilization of substances containing nucleophilic groups. The high surface area system is formed by aggregating the latex particles or by bonding the latex particles to a porous support of high surface area. The latex particles contain groups such as oxirane groups that react with the nucleophilic groups. The substances containing nucleophilic groups may be enzymes or proteins such as albumin, immunoglobulins, blood-clotting factors, cell-membrane proteins or peptide hormones. The high surface area system may be used as a sorbent in removing pollutants, as a stationary phase in organic synthesis such as peptide synthesis, and in the therapeutic treatment of a patient.

High surface area support having bound latex particles containing

What is disclosed is a method for preparing carrier systems, adaptable to the covalent bonding thereto of biologically active materials, consisting of a known solid support material having a surface coated with a polymer containing functional groups suitable for covalent bonding, by coating the support material with a film-forming polymer dispersion and a non-film-forming polymer dispersion, suitably in admixture, at least one of the two polymer dispersions containing a polymer having functional groups suitable for covalent bonding with a biologically active material.

Method for making carrier systems for biologically active materials

A copolymer, adaptable to use as a bead-like carrier material for affinity chromatography, of a hydrophilic allyl monomer of the formula CH.sub.2 .dbd.CH--CH.sub.2 --O--R--X.sub.n, wherein n is 0 and R is hydrogen, or n is a whole number from 1 to 5 and R is an (n+1)-valent aliphatic hydrocarbon group, and X is the same or different hydrophilic hydroxylic group, which copolymer is crosslinked by units of a polyfunctional comonomer in which at least two ethylenically unsaturated groups are linked by a chain containing a carbonamide or ether group.

Crosslinked, activatable, hydrophilic carrier beads

Methods for adsorbing a protein, for example an enzyme, onto an insoluble, solid, macroporous, small-particle support by washing said support with an aqueous solution of the protein containing an electrolyte in an ionic strength of at least 0.15 mole/liter and crosslinking said protein, before, during, or after such adsorption, with a coupling component present in aqueous electrolyte-containing solution.

Method for immobilizing dissolved proteins

Polymer particles dispersible to form a latex and latices of such polymer particles, said particles being adaptable to the fixing or bonding thereto of a biologically active substance and having a core-shell construction wherein the polymer material of the core determines the stability of the form of the latex particles and their redispersibility, and the material of the shell

Polymer particles and latices thereof for the immobilization of

Antigenic substances having amino and/or carboxyl groups are bound covalently, with preservation of their electrochemical state, that is with preservation of the amino and/or carboxyl groups, to a particulate carrier which is not decomposable in the body of a vertebrate; such substances are introduced as an antigen into the body of a vertegrate to provoke an immune response of high specificity and long duration.

Method for inducing antibody formation

The invention relates to diagnostic agents for the rapid diagnostic detection of blood in excretions and body fluids, consisting essentially of a solid support which is charged with a chromogenic substance as indicator and impregnated with an acrylic and/or methacrylic resin.

Rapid diagnostic agents

Packaged diagnostic test strip comprising a first or front sheet having at least one aperture therein, said aperture or apertures having at least one included angle equal to or less than 90 degrees; a diagnostic test strip under said front sheet and extending under said aperture or apertures, said test strip carrying a diagnostic reagent; a second or back sheet supporting said test strip in layered arrangement therewith and with said front sheet, said back sheet having at least one flap-covered aperture therein in that region thereof corresponding with the location of said aperture or apertures in said front sheet, whereby when the flap is open said test strip is exposed thereunder; and a closure flap at least partially extending over said front sheet and having closure means thereon for covering said aperture or apertures in said front sheet when in closed position.

Packaged diagnostic test strip

What is disclosed is a pearl polymer product, a method of making the same, and the use thereof as a carrier for biologically active materials, said product being composed of hollow pearl particles having a diameter from 5 to 1000 microns and consisting of a cross-linked polymer which is a cross-linked homopolymer or copolymer comprising at least one member selected from the group consisting of acrylamide, methacrylamide, methylene-bis-acrylamide and methylene-bis-methacrylamide, said polymer containing at least 5 percent by weight of a monomer having at least two carbon-carbon double bonds which are activated by a neighboring phenyl group or carbonyl group, said polymer further being of a composition such that x+3y.gtoreq.40, where x is the percent by weight in the polymer of acrylamide and methacrylamide and y is the percent by weight in the polymer of methylene-bis-acrylamide and methylene-bis-methacrylamide.

Pearl polymer containing hollow pearls

Water-swellable, cross-linked, bead copolymers of (A) an ethylenically unsaturated monomer having a carboxylic acid anhydride, glycidyl or succinimide group, (B) a comonomer having at least two radical-polymerizable carbon double bonds, and (C) a radical-polymerizable water-soluble comonomer are disclosed to be excellent carriers capable of bonding with biologically active substances such as enzymes, enzyme substrates, inhibitors, hormones, antibiotics, antigens, and peptides.

Water-swellable, bead copolymer

Methods for bonding a polypeptide to a macro-molecular carrier compound by irradiating an aqueous solution of the polypeptide, in the absence of oxygen and in the presence of said carrier compound, with ultraviolet light in the optional presence of a photosensitizer or with visible light in the presence of a photosensitizer and an organic peroxide.

Growing community of inventors

Mainz, Germany

Dieter Kraemer