Promega Corporation

Molecular Devices Corporation

Promeaga Corporation

Dieter Klaubert

Keith V Wood

Mark McDougall

Chad Zimprich

Georgyi V Los

William Daily

Lance P Encell

Monika G Wood

Poncho Meisenheimer

Wenhui Zhou

James J Cali

James Unch

Aldis Darzins

Erika Hawkins

John William Shultz

Robert F Bulleit

Carolyn Woodroofe Hitko

Harry Tetsuo Uyeda

Michael P Valley

Martha A O'Brien

Susan Frackman

Daniel J Simpson

Tonny Johnson

Samuel Kin Sang Ho

Sergei Saveliev

Jianquan Liu

Michael Scurria

Thomas A Kirkland

Paul Otto

Rachel Friedman Ohana

Nidhi Nath

Marjeta Urh

Robin Hurst

Zhenjun Diwu

Hui Wang

Donna M Leippe

Jolanta Vidugiriene

Fen Huang

Sarah Duellman

Ji Zhu

Guoliang Yi

Mary Sobol

Mike Scurria

Martin Kirk

Mary P Hall

Brock Binkowski

Matthew B Robers

Michael R Slater

Cesear Corona

John W Schultz

Helene A Benink

Samuel K Ho

Erica Hawkins

Martha O'BRIEN

Donna Leippe

Chad Zimbrich

Martha O'Brian

Bill Daily

Robet F Bulleit

Chard Zimprich

Mark Mcdougall

The invention provides surfactant compounds of formulas I-IX, which can be used in methods for aiding the solubilization, digestion, preparation, analysis, and/or characterization of biological material, for example, proteins or cell membranes. The compounds can also aid in the recovery of peptides generated during protein digestion, particularly for in-gel digestion protocol. Additionally, the compounds can improve enzymatic protein deglycosylation without interfering with downstream sample preparation steps and mass spectrometric analysis. The compounds can be specifically useful as digestion aids that can be decomposed by an acid, by heat, or a combination thereof. Decomposition of the surfactants allows for facile separation from isolated samples, and/or allows for analysis of the sample without interfering with the sensitivity of various analytical techniques.

Cleavable surfactants

An isolated polynucleotide encoding a modified luciferase polypeptide and substrates. The OgLuc variant polypeptide has at least 60% amino acid sequence identity to SEQ ID NO: 1 and at least one amino acid substitution at a position corresponding to an amino acid in SEQ ID NO: 1. The OgLuc variant polypeptide has at least one of enhanced luminescence, enhanced signal stability, and enhanced protein stability relative to the corresponding polypeptide of the wild-typeluciferase.

Oplophorus-derived luciferases, novel coelenterazine substrates, and methods of use

A mutant hydrolase optionally fused to a protein of interest is provided. The mutant hydrolase is capable of forming a bond with a substrate for the corresponding nonmutant (wild-type) hydrolase which is more stable than the bond formed between the wild-type hydrolase and the substrate. Substrates for hydrolases comprising one or more functional groups are also provided, as well as methods of using the mutant hydrolase and the substrates of the invention. Also provided is a fusion protein capable of forming a stable bond with a substrate and cells which express the fusion protein.

Covalent tethering of functional groups to proteins

The present invention provides compositions and methods for capture and identification of the cellular targets of a bioactive agent. In particular, provided herein are bioactive agents tethered to capture ligand, cellular targets (optionally tagged with a reporter), capture proteins (optionally present as capture fusions), surfaces (e.g., displaying, capture ligands, capture proteins, or capture fusions), and methods of capturing and identifying the cellular targets of a bioactive agent therewith.

Compositions and methods for capture of cellular targets of bioactive agents

The present invention provides an assay for detection of oxidized glutathione (GSSG).

Oxidized glutathione assay

A method to detect the presence or amount of at least one molecule in a sample which employs a derivative of luciferin or a derivative of a fluorophore is provided. Compounds and compositions for carrying out the methods of the invention are also provided.

Luminogenic and fluorogenic compounds and methods to detect molecules or conditions

A mutant hydrolase optionally fused to a protein of interest is provided. The mutant hydrolase is capable of forming a bond with a substrate for the corresponding nonmutant (wild-type) hydrolase which is more stable than the bond formed between the wild-type hydrolase and the substrate and has at least two amino acid substitutions relative to the wild-type hydrolase. Substrates for hydrolases comprising one or more functional groups are also provided, as well as methods of using the mutant hydrolase and the substrates of the invention. Also provided is a fusion protein capable of forming a stable bond with a substrate and cells which express the fusion protein.

Method of immobilizing a protein or molecule via a mutant dehalogenase that is bound to an immobilized dehalogenase substrate and linked directly or indirectly to the protein or molecule

The invention provides compounds, compositions, methods, substrates, and kits useful for analyzing the metabolic activity in cells, tissue, and animals and for screening test compounds for their effect on cytochrome P450 activity. In particular, a one-step and two-step methods using luminogenic molecules, e.g. luciferins or coelenterazines, that are cytochrome P450 substrates and that are also bioluminescent enzyme, e.g., luciferase, pro-substrates are provided. The present method further provides a method for stabilizing and prolonging the luminescent signal in a luciferase-based assay using luciferase stabilizing agents such as reversible lucifetrase inhibitors.

Luminescence-based methods and probes for measuring cytochrome P450 activity

The present invention provides compositions and methods for capture and identification of the cellular targets of a bioactive agent. In particular, provided herein are bioactive agents tethered to capture ligand, cellular targets (optionally tagged with a reporter), capture proteins (optionally present as capture dimers), surfaces (e.g., displaying, capture ligands, capture proteins, or capture dimers), and methods of capturing and identifying the cellular targets of a bioactive agent therewith.

Covalent tethering of functional groups to proteins and substrates therefor

A method to detect the presence of amount of at least one molecule in a sample which employs a derivative of luciferin or a derivative of a fluorophore is provided. Compounds and compositions for carrying out the methods of the invention are also provided.

The present invention provides compounds and methods for assaying redox state of metabolically active cells and methods for assaying enzyme activity and/or metabolite level by coupling to redox defining co-factor NAD(P)/NAD(P)H measurement.

Compounds and methods for assaying redox state of metabolically active cells and methods for measuring NAD(P)/NAD(P)H

Growing community of inventors

Arroyo Grande, CA, United States of America

Dieter Klaubert