Hoechst Aktiengesellschaft

Dieter Brauer

Klaus Bartsch

Susanne Grabley

Alfred Puhler

Wolfgang Wohlleben

Gerhard Wohner

Rudiger Marquardt

Walter Arnold

Eckhard Strauch

Renate Alijah

Gunter Donn

Selection of Streptomyces viridochromogenes DSM 4112 for resistance to phosphinothricyl-alanyl-alanine (PTT) results in PTT-resistance selectants. The DNA fragment which carries the phosphinothricin(PTC)-resistance gene is obtained from the total DNA of these selectants by cutting with BamHI, cloning of a fragment 4.0 kb in size, and selection for PTT resistance. This gene is suitable for the production of PTC-resistant plants, and as a resistance marker and for the selective N-acetylation of the L-form of racemic PTC.

Phosphinothricin-resistance gene, and its use

Phosphinothricin (PTC) selection of bacteria which are not fungus-like yields PTC-resistant selectants. The DNA fragment which carries the resistance gene is obtained from the complete DNA of these selectants by constructing a gene bank and screening for chemical modification of PTC. The resistance gene can be localized to a fragment which is 2 kb in size, and selection for PTC resistance. This gene is suitable for producing PTC-resistant plants and propagation material thereof, and as a resistance marker as well. Microorganisms which contain this PTC-resistance gene can be used in sewage treatment plants.

Phosphinothricin-resistance gene

Growing community of inventors

Florsheim am Main, Germany

Dieter Brauer