Boehringer Mannheim Gmbh

Dieter Berger

Wolfgang Werner

Gunter Frey

Manfred Kuhr

Werner Guthlein

Franz Braun

Wolfgang-Reinhold Knappe

Peter Vogel

Hans-Peter Braun

Walter Rittersdorf

Hans Lange

Heinz Kurt Macho

Peter Rieckmann

Bernward Sojka

Erich Weiss

Manfred Bleisteiner

Henry M Grage, Jr

Mark T Skarstedt

Hans-Rudiger Murawski

Elke Biegel

Robert Lorenz

On a carrier layer there are arranged several test layers which are at least partly in fluid contact with one another, enabling liquid exchange. On the carrier layer are arranged an application zone, a detection zone for the production of a detectable signal characteristic of the analytical determination, which contains at least one said test layer which is a reaction layer, and an absorption zone with a test layer which is an absorptive layer of an absorbent material, wherein the reaction layer and absorption zone are positioned next to each other. Between the application and the absorption zone is a capillary-active transport path on the carrier layer which connects the application zone and the absorption zone. The reaction layer is arranged parallel to the transport path between the application zone and the absorption zone in such a manner that it is in liquid contact with a liquid transported in the transport path. The absorption zone therefore absorbs excess liquid and permits dosing the reaction layer with a reproducible amount of sample.

Test carrier and method for the analytical determination of components

A device for separating plasma or serum from whole blood and analyzing the same, wherein the device includes a layer of glass fibers with an average diameter of from 0.2 to 5 microns and having a density of from 0.1 to 0.5 g./cm.sup.3 and an adsorption volume at least twice that of the plasma or serum to be separated and, preferably, more than 3.3 times the volume.

Device for separating plasma or serum from whole blood and analyzing the

A test support for support-bound tests for the determination of components of fluids, especially of human and animal body fluids. The test support is composed of a plurality of layers, and the joining of the layers is accomplished at least partially by sewing.

Test carrier

Esters and agents including the esters, useful as substrates for esterolytic and/or proteolytic enzymes, said esters being compounds of the general formula: ##STR1## in which R'.sub.1, R'.sub.2, R'.sub.3 and R'.sub.4, which may be the same or different, are hydrogen atoms or lower alkyl, lower alkoxy, alkylamino or dialkylamino radicals or in which two adjacent substituents can also represent a fused benzene ring, A is a residue of an amino acid or of a peptide and B is a nitrogen protective group conventional in peptide chemistry or derived therefrom and the method of preparation and use.

Agent for the determination of esterolytic and/or proteolytic enzymes

Esters and agents including the esters, useful as substrates for esterolytic and/or proteolytic enzymes, said esters being compounds of the general formula: ##STR1## in which R'.sub.1, R'.sub.2, R'.sub.3 and R'.sub.4, which may be the same or different, are hydrogen atoms or lower alkyl, lower alkoxy, alkylamino or dialkylamino radicals or in which two adjacent substituents can also represent a fused benzene ring, A is a residue of an amino acid or of a peptide and B is a nitrogen protective group conventional in peptide chemistry or derived therefrom; and the method of preparation and use.

A composition and process for permitting the separation of plasma or serum from whole blood. The composition includes glass fibers having an average diameter of from 0.2.mu. to 5.mu. and a density of 0.1 g/cm.sup.2 to 0.5 g/cm.sup.2. The process includes the steps of slowly trickling whole blood onto one side of a layer composed of the composition of glass fibers, whereby plasma or serum separated from the blood becomes available at another side of the layer. The total volume of the plasma or serum separated from the blood is limited to at most 50% of the absorption volume of the glass fiber layer.

Process and composition for separating plasma or serum from whole blood

This invention relates to new amino acid and peptide esters of leuko-indoaniline compounds and to a process for their preparation. In additional aspect, the invention relates to compositions containing such compounds for the detection of proteolytic enzymes and to methods for detecting such enzymes.

Amino acid and peptide esters of leuko-indoaniline compounds and

Azo dyestuff for use as a chromogen in detecting leukocytes

Diagnostic agent for the detection of leukocytes in body fluids, comprising an absorbent carrier which is impregnated with a chromogen and a buffer substance, wherein the chromogen is a sulfonphthalein ester of the formula ##STR1## wherein R.sub.1 is a carboxylic acid residue optionally substituted by halogen or lower alkoxy; or is an amino acid or peptide residue having a nitrogen protective group;

Diagnostic agent for the detection of leukocytes and chromogens useful

Diagnostic agent for the detection of component materials, in liquids and method of production. The diagnostic agent includes a water resistant film composed of a film-former and contains a film opener in the form of fine, insoluble inorganic or organic particles.

Diagnostic agent for the detection of component materials in liquid and

This invention relates to diagnositc agents and methods for the detection of leukocytes in body fluids. In additional aspect, the invention provides a process for the production of such diagnostic agents.

Diagnostic agents for the detection of leukocytes in body fluids

Novel diagnostic composition for the detection of leukocytes in body fluids, comprising an absorbent carrier impregnated with a buffer substance and a chromgen, wherein the chromogen is an azo dyestuff ester of the formula

Diagnostic composition for the detection of leukocytes and chromogens

The present invention provides a diagnostic agent for the detection of proteolytic enzymes, comprising an absorbent carrier, a film layer, a powder mixture, a lyophilisate, a solution or a reagent tablet, containing at least one chromogen and an appropriate buffer substance, wherein the chromogen used is an indoxyl- and/or thioindoxyl-amino acid ester and/or peptide ester of the general formula: ##STR1## wherein R.sub.1, R.sub.2, R.sub.3 and R.sub.4, which can be the same or different, are hydrogen or halogen atoms, lower alkyl, lower alkoxy, aryl, aralkyl, aralkoxy, hydroxyl, carboxy, carboxy lower alkoxy, aralkoxycarbonyl, aralkyloxycarbonyl lower alkoxy, nitro or lower acylamino radicals, whereby two adjacent substituents can also represent an optionally halogenated fused benzene ring, X is a sulphur atom or an imino group optionally substituted by a lower alkyl, aryl, aralkyl or acyl radical, A is an amino acid or peptide residue and B is a nitrogen protective group conventional in peptide chemistry or derived therefrom. The present invention also provides new compounds of the above-given general formula and processes for the preparation thereof. Furthermore, the present invention is concerned with the use of the compounds of the above-given general formula for the production of diagnostic agents for the detection of proteolytic enzymes.

Diagnostic agents for the detection of proteolytic enzymes

Pre-coated microscope slides are produced on a large scale by spraying a dyestuff solution on to microscope slides which are passed at a constant speed under a spray nozzle, the pattern of which is kept rectangular by means of a mask. Apparatus for carrying out the process includes a conveyor belt, a regulatable spraying device positioned above the conveyor belt and a mask positioned between the spraying device and the conveyor belt.

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Viernheim, Germany

Dieter Berger